RENT tsS1.1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: rat endometrium
• 包装: tube of 5 μg 92092515-DNA-5UG; pkg of vial of cells 92092515-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Cuboidal
• 产品: Not specified
• 受体: Glucocorticoid
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed

细胞系描述

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43 markers. The transforming agent is a ts mutant of SV 40 T-antigen (tsA58).

培养基

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.

其他说明

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