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形狀
lyophilized powder
比活性
≥100 units/mg protein
成份
Protein, ≥50% biuret
運輸包裝
wet ice
儲存溫度
−20°C
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應用
来自 Sigma 的酶已在各种研究中用于胆汁样品的分析。
生化/生理作用
该酶是一种 N-末端亲核 (Ntn) 水解酶,催化酰胺键水解,从类固醇核心释放甘氨酸/牛磺酸部分,最终生成非结合胆汁酸。已证明可氧化巯基的药物(例如,对汞苯甲酸盐、碘乙酰胺、Hg 2 + 、Cu 2 + 和 Cd 2 + )可强烈抑制 产气荚膜梭菌的胆盐水解酶 (BSH) 活性 。
單位定義
一个单元将在37°C、pH 5.6 条件下5 min 内将 1.0μmol 甘胆酸水解为甘氨酸和胆酸。
外觀
部分纯化的冻干粉末,含有缓冲盐和稳定剂
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Biochemistry, 44(15), 5739-5748 (2005-04-13)
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction
Effects of Quercetin and Rutin on Serum and Hepatic Lipid Concentrations, Fecal Steroid Excretion and Serum Antioxidant Properties
Journal of Health Science, 46(4), 229-240 (2000)
Bile salt hydrolase activity in probiotics.
Applied and environmental microbiology, 72(3), 1729-1738 (2006-03-07)
Archives of microbiology, 191(7), 571-581 (2009-05-14)
The virB genes coding type IV secretion system are necessary for the intracellular survival and replication of Brucella spp. In this study, extracellular proteins from B. abortus 2308 (wild type, WT) and its isogenic virB10 polar mutant were compared. Culture
Journal of agricultural and food chemistry, 56(18), 8360-8364 (2008-08-30)
The interest in efficient intestinal delivery of health-promoting substances is increasing. However, the delivery of vulnerable substances such as enzymes requires specific attention. The transit through the stomach, where the pH is very low, can be detrimental to the enzymatic
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