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Merck

C3306

Sigma-Aldrich

氯化钙 二水合物

for molecular biology, ≥99.0%

别名:

CaCl2.2H2O, Calcium chloride, Calcium dichloride

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About This Item

经验公式(希尔记法):
CaCl2 · 2H2O
CAS号:
分子量:
147.01
EC號碼:
MDL號碼:
分類程式碼代碼:
12352302
PubChem物質ID:
NACRES:
NA.31

等級

for molecular biology

化驗

≥99.0%

形狀

powder

mp

176 °C (dec.) (lit.)

溶解度

H2O: 294 mg/mL, clear, colorless

異物活動

DNase, RNase, protease, none detected

SMILES 字串

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI 密鑰

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

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應用

氯化钙二水合物用于:
制备合格大肠杆菌用于转化(118)
用于制备聚磷酸钙纳米球和诱导碱性磷酸酶(119)
复性缓冲液的成分,用于金黄色葡萄球菌粗酶提取物(120)酶谱

生化/生理作用

氯化钙与质粒一起用于微生物的热休克转化。带正电的钙离子与DNA磷酸盐和脂多糖(LPS)中带负电的磷酸化脂质A形成阳离子桥,有助于DNA转化进微生物。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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Preparation of calcium competent Escherichia coli and heat-shock transformation
Chang AY, et al.
Journal of Experimental Microbiology and Immunology, 1, 22-25 (2017)
Hui Sun Leong et al.
Nature communications, 5, 3947-3947 (2014-05-24)
Non-coding RNAs (ncRNAs) are frequent and prevalent across the taxa. Although individual non-coding loci have been assigned a function, most are uncharacterized. Their global biological significance is unproven and remains controversial. Here we investigate the role played by ncRNAs in
Bradley T Estes et al.
Nature protocols, 5(7), 1294-1311 (2010-07-03)
The ability to isolate, expand and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, as well
Zhijian Zhao et al.
Scientific reports, 10(1), 4399-4399 (2020-03-12)
The retina decomposes visual stimuli into parallel channels that encode different features of the visual environment. Central to this computation is the synaptic processing in a dense layer of neuropil, the so-called inner plexiform layer (IPL). Here, different types of
Susan E Logue et al.
Nature protocols, 4(9), 1383-1395 (2009-09-05)
Apoptosis is a mode of programmed cell death that is widely used to eliminate cells during development, tissue homeostasis, infection or in response to injury. Alterations to the plasma membranes of apoptotic cells trigger recognition and engulfment of such cells

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