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生物来源
Electrophorus electricus
类型
Type V-S
表单
lyophilized powder
比活
≥1,000 units/mg protein
分子量
280 kDa
组成
Protein, ≥45% biuret
pI
~5.3
溶解性
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
储存温度
−20°C
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一般描述
分子量： 280 kDa
等电点： 5.5
消光系数： E1%= 18.0（280 nm）
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体，每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
等电点： 5.5
消光系数： E1%= 18.0（280 nm）
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体，每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
应用
电鳗乙酰胆碱酯酶已用于：
- 乙酰胆碱酯酶活性检测
- 研究其对爪蟾胚胎海马体细胞的影响
- 研究乙酰胆碱抑制剂对突触发育和可塑性的影响
生化/生理作用
体内乙酰胆碱的主要降解酶。 将乙酰胆碱 + H2O 转化为胆碱+乙酸。
乙酰胆碱酯酶(AChE)的活性表现于其可降解神经肌肉接头和胆碱能神经元突触间隙处的乙酰胆碱。该酶参与一氧化氮信号通路。
注意
对于稀释溶液（<1 mg/ml），加入 1mg/ml BSA 以稳定。在稀释的中性磷酸盐缓冲液中含有至少 1mg 蛋白质/ml 的溶液在冷藏至少 6 个月后是稳定的。
单位定义
一个单元将在 pH8.0，37℃下，每分钟将 1.0 μmole 乙酰胆碱水解成胆碱和乙酸盐。
外形
含 Tris 缓冲盐的冻干粉
分析说明
使用乙酰胆碱作为底物获得的活性是用丁基胆碱获得的活性的 30-100 倍，使用来自电鳗的乙酰胆碱酯酶。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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