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螢光
λex 546 nm; λem 579 nm
運輸包裝
wet ice
儲存溫度
2-8°C
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一般說明
Baseclick EdU Flow Cytometry Kit为直接检测DNA合成提供了替代BrdU和[3H]thymidine检测的出色方法。EdU(5-乙炔基-2'.-脱氧尿苷)是一种胸苷核苷类似物,在活性DNA合成期间掺入到DNA之中。与BrdU检测法不同,EdU Flow Cytometry Assay不依赖抗体,因而不需进行DNA变性来检测掺入的核苷。恰恰相反,EdU Flow Cytometry Kit利用点击化学法来检测不同种类的染料荧光示值。此外,该试剂盒的实验方案经过简化,减少了总步骤数同时大幅缩减了总耗时。简单的点击化学法检测步骤可在30分钟内完成,在结果的内容和情境丰富程度方面,堪比多重分析方法。
應用
流式细胞术
訊號詞
Danger
危險分類
Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Cell, 175(2), 442-457 (2018-10-06)
Antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP) critically contribute to the efficacy of anti-tumor therapeutic antibodies. We report here an unexpected finding that macrophages after ADCP inhibit NK cell-mediated ADCC and T cell-mediated cytotoxicity in breast cancers and lymphomas.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
相关内容
Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.
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