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Merck

B6404

Supelco

BCIP®/NBT 溶液,预混合

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About This Item

分類程式碼代碼:
41105333
NACRES:
NA.32

應用

BCIP/NBT 溶液,经过预混合后:
  • 作为底物用于斑点印迹显影
  • 作为底物用于在免疫组织化学分析中呈现碱性磷酸酶
  • 在蛋白质印迹分析中检测特异性免疫复合物

其他說明

0.48mM NBT,0.56mM BCIP,10mM Tris 和 59.3mM MgCl2,pH 约 9.2

基底

用于在蛋白质印迹中显现碱性磷酸酶缀合物的底物。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach
Alcocer M J C, et al.
Journal of Molecular Biology, 343(3), 759-769 (2004)
Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent
Tsai R K, et al.
Experimental Eye Research, 90(5), 537-545 (2010)
Sandra Fernandes Arruda et al.
Nutrients, 12(5) (2020-05-28)
The Brazilian savanna fruit, tucum-do-cerrado (Bactrissetosa Mart.) reduces hepatic hepcidin levels. Therefore, we investigated the effect of tucum-do-cerrado on the TfR/HFE and/or BMP/HJV/SMAD and JAK/STAT pathways, in normal and excess iron conditions. Rats were treated with: control diet (CT); control
Neuroprotective effects of recombinant human granulocyte colony-stimulating factor (G-CSF) in neurodegeneration after optic nerve crush in rats
Tsai R K, et al.
Experimental Eye Research, 87(3), 242-250 (2008)
Willie Lin et al.
Oncotarget, 9(2), 1992-2001 (2018-02-09)
CD34 is a transmembrane phosphoglycoprotein used to selectively enrich bone marrow in hematopoietic stem cells for transplantation. Treating rats with CD34

商品

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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