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![N-[2-[2-[2-(2-叠氮乙氧基)乙氧基]乙氧基]乙基]生物素胺](/deepweb/assets/sigmaaldrich/product/structures/120/306/c9779b03-3754-4ad6-8eef-b07209e113ce/640/c9779b03-3754-4ad6-8eef-b07209e113ce.png)
描述
Sulfhydryl specific, water soluble biotinylation reagent
形狀
powder
溶解度
H2O: 10 mg/mL
儲存溫度
2-8°C
SMILES 字串
ICC(=O)NCCOCCOCCNC(=O)CCCCC1SCC2NC(=O)NC12
InChI
1S/C18H31IN4O5S/c19-11-16(25)21-6-8-28-10-9-27-7-5-20-15(24)4-2-1-3-14-17-13(12-29-14)22-18(26)23-17/h13-14,17H,1-12H2,(H,20,24)(H,21,25)(H2,22,23,26)
InChI 密鑰
LWGLKVINXNMNJX-UHFFFAOYSA-N
相关类别
一般說明
生物素聚环氧乙烷（PEO）碘乙酰胺是巯基（硫醇）特异性生物素化试剂。
應用
生物素聚氧化乙烯碘乙酰胺已被用作巯基反应试剂，以证明人乳头瘤病毒 16 的衣壳蛋白 L1 中存在游离巯基。已用于 ELISA（酶联免疫吸附试验）竞争和牵出试验的蛋白生物素化。
- 包含一个 12 原子亲水间隔区。
- pH 7.5-8.5 通常与巯基偶联
- 在许多蛋白质组学类型应用（如肽图和质谱）中，半胱氨酸标记尤其有用。
生化／生理作用
生物素聚环氧乙烷（PEO）碘乙酰胺基团在pH 7.5-8.5下与还原的硫醇发生特异性反应。含有巯基的肽和小分子也可用该试剂标记。生物素PEO碘乙酰胺能够标记半胱氨酸残基并与蛋白质中的巯基偶联，从而使其可用于蛋白质组学、肽图分析、磷酸肽分析和质谱分析。
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Gloves
Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L1.
Ishii Y, et al.
Virology Journal, 4, 110-110 (2007)
Beta-peptides with improved affinity for hDM2 and hDMX.
Harker EA, et al.
Bioorganic & Medicinal Chemistry, 17, 2038-2046 (2009)
A thermodynamic model for Nap1-histone interactions.
Andrews AJ, et al.
The Journal of Biological Chemistry, 283, 32412-32418 (2008)
Eric A Berg et al.
Cold Spring Harbor protocols, 2020(1), pdb-pdb (2020-01-04)
There are several techniques for biotinylating antibodies, from the most basic (using NHS-ester biotin to label primary amines) to more complex experiments (modifying sulfhydryls and carbohydrates). Biotinylation of free sulfhydryls, described here, can be effectively mediated using haloacetyl biotin derivatives.
Barbara Roman et al.
Journal of molecular and cellular cardiology, 144, 87-96 (2020-05-23)
In cardiomyocytes, there is microRNA (miR) in the mitochondria that originates from the nuclear genome and matures in the cytoplasm before translocating into the mitochondria. Overexpression of one such miR, miR-181c, can lead to heart failure by stimulating reactive oxygen
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