推荐产品
表单
liquid
包装
pkg of 250 mL (AC-60250 (HighPrep™ PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep™ PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep™ PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep™ PCR (500 mL)))
储存条件
do not freeze
储存温度
2-8°C
一般描述
Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.
应用
HighPrep™ PCR Clean-up System may be used in:
- Post-PCR and post-enzymatic reaction clean-up
- NGS library preparation
- Amplicon size selection for NGS
- Microarrays
- PCR
- Sanger sequencing
- Restriction enzyme digestions, adapter ligations
- Cloning
特点和优势
- High recovery of >100 bp amplicons
- Adaptable to high throughput liquid handling systems
- Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
- No centrifugation or filtration leads to higher and purer yields
储存及稳定性
14 months when stored as directed
法律信息
HighPrep is a trademark of MagBio Genomics, Inc.
免责声明
For Research Use Only. Not for Use in Diagnostic Procedures.
警示用语：
Warning
危险声明
危险分类
Repr. 2
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Eva Baggesgaard Sterndorff et al.
Environmental research, 185, 109449-109449 (2020-04-12)
Activity of the microbial population in clothing causes unpleasant odor and textile deterioration. However, little is known about how the textile microbial community is shaped. In this study, we developed a method for extracting DNA from small amounts of detergent-washed
Shuba Varshini Alampalli et al.
PloS one, 12(12), e0188603-e0188603 (2017-12-09)
India has the third largest number of HIV-1-infected individuals accounting for approximately 2.1 million people, with a predominance of circulating subtype C strains and a low prevalence of subtype A and A1C and BC recombinant forms, identified over the past
The T-shirt microbiome is distinct between individuals and shaped by washing and fabric type
Sterndorff EB, et al.
Environmental Research, 109449-109449 (2020)
Lauren Bowen Reoma et al.
Annals of neurology, 86(6), 878-884 (2019-10-11)
JC virus (JCV) infection is a lytic infection of oligodendrocytes in progressive multifocal leukoencephalopathy; less common forms of central nervous system manifestations associated with JCV infection include granule cell neuronopathy, encephalopathy, and meningitis. Presented is the first case of fatal
Signature of genome wide gene expression in classical swine fever virus infected macrophages and PBMCs of indigenous vis-a-vis crossbred pigs
Sah V, et al
Gene, 144356-144356 (2020)
实验方案
HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.
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