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A9415

Sigma-Aldrich

放线菌素D

from Streptomyces sp., suitable for cell culture, ≥95%

别名:

放线菌素C1, 放线菌素IV, 更生霉素

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About This Item

经验公式（希尔记法）:
C62H86N12O16
CAS号:
50-76-0
分子量:
1255.42
Beilstein:
605235
EC號碼:
200-063-6
MDL號碼:
MFCD00005033
分類程式碼代碼:
51282001
PubChem物質ID:
24891447
NACRES:
NA.76

生物源

Streptomyces sp.

品質等級

300

化驗

≥95%

形狀

powder

技術

cell culture | mammalian: suitable

顏色

red, powder

mp

251-253

溶解度

ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)

抗生素活性譜

neoplastics

作用方式

DNA synthesis | interferes

儲存溫度

2-8°C

SMILES 字串

CC(C)[C@H]1NC(=O)[C@@H](NC(=O)c2ccc(C)c3OC4=C(C)C(=O)C(N)=C(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]6CCCN6C(=O)[C@H](NC5=O)C(C)C)C4=Nc23)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]7CCCN7C1=O

InChI

1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1

InChI 密鑰

RJURFGZVJUQBHK-IIXSONLDSA-N

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相关类别

一般說明

化学结构：肽

應用

放线菌素 D 是一种抑制细胞增殖的抗肿瘤抗生素，具有广泛的应用，包括作为细胞培养中的选择剂，用在抑制 HIV 复制和 PC12 细胞程序性死亡的研究中。 细胞培养应用的使用浓度推荐为 1μg/mL。

生化／生理作用

放线菌素 D 通过与双链 DNA 形成稳定的复合物，抑制 DNA 引发的 RNA 合成并引起 DNA 的单链断裂来抑制细胞的增殖。 研究表明，它是逆转录酶中负链转移步骤的抑制剂。

注意

该产品具有吸湿性和光敏性。 如果在 2-8°C 下避光保存，经 HPLC 测试，可保持至少 15 个月。 未使用的放线菌素 D 稀溶液对光极为敏感，容易吸附到塑料和玻璃上，应丢弃。 但是，浓缩原液的冷冻等分试样至少可以稳定一个月。

準備報告

放线菌素 D 以红色、有光泽的晶体形式出售，可溶于乙腈或丙酮，浓度为 10 mg/mL，溶于 DMSO 中最低浓度为 1 mg/mL。 一些参考文献还显示出在水中的微弱溶解度为 0.5 mg/mL。

其他說明

保存于密闭容器内，置于干燥通风处。置于干燥处保存。

象形圖

Skull and crossbonesHealth hazard
GHS06,GHS08

訊號詞

Danger

危險聲明

H300,H350,H360FD

危險分類

Acute Tox. 2 Oral - Carc. 1B - Repr. 1B

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000375979
0000375979
0000325066
0000327286
089M4017V

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Antonio Rescifina et al.
Journal of medicinal chemistry, 49(2), 709-715 (2006-01-20)
Isoxazolidinyl polycyclic aromatic hydrocarbons (isoxazolidinyl-PAHs) have been synthesized in good yields by 1,3-dipolar cycloaddition methodology promoted by microwave irradiation. The structures of the obtained cycloadducts have been determined by NOE experiments and supported by computational studies at the AM1 level.
Arkady Khoutorsky et al.
Neuron, 78(2), 298-311 (2013-04-30)
Control of protein synthesis is critical for synaptic plasticity and memory formation. However, the molecular mechanisms linking neuronal activity to activation of mRNA translation are not fully understood. Here, we report that the translational repressor poly(A)-binding protein (PABP)-interacting protein 2A
Angela Zarama et al.
PLoS pathogens, 10(3), e1004000-e1004000 (2014-03-15)
Receptors of the signalling lymphocyte-activation molecules (SLAM) family are involved in the functional regulation of a variety of immune cells upon engagement through homotypic or heterotypic interactions amongst them. Here we show that murine cytomegalovirus (MCMV) dampens the surface expression
Rolf Schreckenberg et al.
Antioxidants & redox signaling, 23(15), 1220-1232 (2015-05-16)
Research into right ventricular (RV) physiology and identification of pathomechanisms underlying RV failure have been neglected for many years, because function of the RV is often considered less important for overall hemodynamics and maintenance of blood circulation. In view of
Mohitosh Maiti et al.
Bioorganic & medicinal chemistry letters, 22(4), 1709-1711 (2012-01-20)
Over-expressions of miRNAs are being increasingly linked with many diseases including different types of cancer. In this study, the role of some known small molecular therapeutics has been investigated for their ability to bind with the pre-miRNA target (hsa-mir-155) and
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