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Merck

A9228

Sigma-Aldrich

淀粉葡萄糖苷酶 来源于根霉菌

≥40,000 units/g solid

别名:

1,4-α-D-葡聚糖葡萄糖水解酶, 糖化淀粉酶, 葡萄糖淀粉酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

表单

lyophilized solid

比活

≥40,000 units/g solid

溶解性

0.03 M sodium citrate-phosphate buffer, pH 4.5: soluble 5.0 mg/mL

储存温度

−20°C

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应用

淀粉葡萄糖苷酶用于水解 α-D-葡糖苷。 它可用于啤酒的酿造和面包和果汁的生产。 来自根霉菌属的淀粉葡萄糖苷酶,已被用于研究在 ER 相关的蛋白质降解(ERAD)期间寡糖的裂解。该酶已用于 N-香草基 - 壬酰胺的糖基化,形成具有药理学应用的水溶性组份。灰盖鬼伞菌的菌柄残留物为材料,用淀粉葡糖苷酶处理,测定其糖原含量。

生化/生理作用

淀粉葡萄糖苷酶能够水解寡糖的 α-D-(1-4),α-D-(1-6)和 α-D-(1-3)糖苷键

单位定义

一个单位将在 pH 4.5,55℃ 下,在 3 分钟内,从淀粉中释放 1.0mg 的葡萄糖。

外形

冻干的无盐粉末

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Ramaiah Sivakumar et al.
Biotechnology letters, 29(10), 1537-1548 (2007-07-05)
Enzymatic syntheses of N-vanillyl-nonanamide, 1, glycosides with D-glucose, 2, D-galactose, 3, D-mannose, 4, D-ribose, 5, maltose, 6, and lactose, 7, were carried out using amyloglucosidase from Rhizopus and beta-glucosidase from sweet almond. The latter catalysed the syntheses of N-vanillyl-nonanamide glycosides
Daisuke Sugiura et al.
Annals of botany, 123(4), 715-726 (2018-12-06)
Sink-source imbalance could cause an accumulation of total non-structural carbohydrates (TNC; soluble sugar and starch) in source leaves. We aimed to clarify interspecific differences in how sink-source imbalance and TNC causes the downregulation of photosynthesis among three legume plants. The
Masabumi Komatsu et al.
Physiologia plantarum, 147(2), 159-168 (2012-05-23)
To determine the effects of elevated CO(2) concentration ([CO(2)]) on the temperature-dependent photosynthetic properties, we measured gas exchange and chlorophyll fluorescence at various leaf temperatures (15, 20, 25, 30, 35 and 40°C) in 1-year-old seedlings of the Japanese white birch
Daisuke Sugiura et al.
Plant & cell physiology, 58(12), 2043-2056 (2017-12-08)
To clarify whether excessive accumulation of total non-structural carbohydrate (TNC) causes down-regulation of photosynthesis in Raphanus sativus, we manipulated sink-source balance to alter TNC levels in source leaves and examined its effects on photosynthetic characteristics, whole-plant biomass allocation and anatomical
Metabolic Control of Fruitbody Morphogenesis in Coprinus cinereus
Gooday GW
Basidium and Basidiocarp, 157-173 (1982)

实验方案

本操作程序可用于使用淀粉作为底物测定淀粉葡萄糖苷酶的活性。

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

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