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Merck

A6982

Sigma-Aldrich

HydroMatrix细胞培养多肽支架

mixture, powder

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About This Item

分類程式碼代碼:
12352207

生物源

synthetic

品質等級

形狀

powder

儲存溫度

−20°C

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應用

HydroMatrix 已被证明可在许多细胞类型培养中促进细胞生长和迁移,包括神经干细胞、神经元、胶质细胞、星形胶质细胞、成纤维细胞和角质形成细胞。

成分

HydroMatrix 是一种肽纳米纤维三维支架,可促进细胞生长和迁移。当使用高温或高离子强度时,它利用从流体前体自组装成高度交联的肽水凝胶的特定肽。通过诱导从溶液到凝胶的快速转化,生成肽纳米纤维支架。调整 HydroMatrix 溶液的浓度,研究人员可以控制三维架构的灵活性,以满足其应用的特定需求。

注意

该冻干品置于 -20℃ 下,在该温度下可稳定多年。水凝胶应储存于 -70°C,使有效期最大化。1%(w/v)储备溶液应储存在 -20°C,并可稳定 3-6 个月。

準備報告

该产品以冻干粉形式提供。加入无菌水可以制备 10mg/mL 的 1%(w/v)储备溶液,得到刚性凝胶。0.5%(w/v)溶液将产生更柔软的凝胶,更适合细胞迁移和血管生成。0.15%-0.25% 范围内的较低浓度更适用于神经元等多种细胞类型。在水溶液中,HydroMatrix 的 pH 值为 2.5,如果没有事先添加缓冲液或培养基,则不应直接添加到细胞中。一旦形成水凝胶,不要试图进一步混合凝胶,因为这有可能破坏水凝胶的完整性。操作细胞时应小心谨慎,以免破坏支架。

法律資訊

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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