A5106

Anti-ABI1 兔抗

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 别名: Anti-Abelson interactor 1, Anti-Abl-interactor protein 1, Anti-Nap1 binding protein NAP1BP, Anti-eps8 binding protein, Anti-interactor protein AblBP4, Anti-spectrin SH3 domain binding protein 1 SSH3BP1
• UNSPSC代码: 12352203
• NACRES: NA.41

属性

• 生物来源: rabbit
• 偶联物: unconjugated
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 表单: buffered aqueous solution
• 分子量: antigen ~65 kDa
• 种属反应性: human, canine (predicted), mouse (predicted), rat
• 增强验证: recombinant expression; Learn more about Antibody Enhanced Validation
• 浓度: ~1.5 mg/mL
• 技术: western blot: 0.5-1.0 μg/mL using A431 cell lysate; western blot: 0.5-1.0 μg/mL using extract of rat brain cytosolic fraction (S1)
• UniProt登记号: Q8IZP0
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... ABI1(10006) ; mouse ... Abi1(11308) ; rat ... Abi1(79249)

说明

一般描述

Abelson interactor 1 (Abi1) protein, a 65 kD-substrate of the eponymous Abelson non-receptor tyrosine kinase (c-Abl) is expressed mainly in the central nervous system. This gene is located on human chromosome 10p12.

Abi 1 is an adaptor protein that interacts with Wave2 to regulate actin polymerization.

免疫原

synthetic peptide corresponding to amino acids 163-180 of human ABI1, conjugated to KLH. The corresponding sequence is identical in several species including mouse, rat, and dog.

应用

Anti-ABI1 antibody produced in rabbit has been used in western blot analysis and immunofluorescence.

Rabbit anti- ABI1 antibody can be used for immunoblotting assays at a concentration of 0.5-1.0μg/ml using extracts from A431 and rat brain cells.

生化/生理作用

Abelson interactor 1 (Abi1) is essential for the formation and activation of WAVE2 signaling complex. ABI1 and WAVE are essential for Rac-dependent membrane protrusion and macropinocytosis, whereas ABI1 and N-WASP regulate actin-based vesicular transport, EGF receptor endocytosis, and EGFR and transferrin receptor (TfR) cell surface distribution. ABI1 has been shown to act as dual regulator of WAVE and neural Wiskott-Aldrich syndrome protein (N-WASP) specific activities. ABI1 is an essential component of this signaling complex. It positively regulates WAVE activity and connects WAVE to Rac through assembly of the WAVE-ABI1-Nap1-PIR121 complex. Abi1 modulates actin reorganization and lamellipodia formation.

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable
• 个人防护装备: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)