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Merck

A1935

Sigma-Aldrich

光敏生物素 乙酸盐

For labeling DNA probes for hybridizations

别名:

N-(4-叠氮-2-硝基苯基)-N′-(3-生物素基氨丙基)-N′-甲基-1,3-丙二胺 乙酸盐, 生物素{3-[3-(4-叠氮-2-硝基苯胺)-N-甲基丙氨基]丙酰胺} 乙酸盐

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About This Item

经验公式(希尔记法):
C23H35N9O4S · C2H4O2
CAS号:
分子量:
593.70
MDL號碼:
分類程式碼代碼:
12352200
PubChem物質ID:
NACRES:
NA.52

等級

for molecular biology

品質等級

化驗

≥95%

形狀

film or powder

溶解度

H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)

儲存溫度

−20°C

SMILES 字串

CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]

InChI

1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1

InChI 密鑰

FFBLNTOMOSLSQM-AYEYRVMASA-N

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一般說明

光生物素乙酸盐由生物素组成,通过带电荷连接臂,与光反应性芳基叠氮化物基团结合。

應用

光生物素乙酸盐用于标记DNA探针,进行DNA−DNA杂交 确定基因组相关性。

生化/生理作用

光生物素乙酸盐能够标记单链DNA和线性或超螺旋双链DNA。
光生物素是可光活化的生物素类似物,主要用于标记DNA、RNA和蛋白。光生物素制备的探针,可检测低至0.5 pg的目标DNA。

注意

光敏。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Chang Soo Lee et al.
International journal of systematic and evolutionary microbiology, 57(Pt 8), 1775-1779 (2007-08-09)
A novel thiosulfate-oxidizing bacterium, designated strain LCS2(T), was isolated from a biofilm on sulfur particles used in an autotrophic denitrification process. The strain was found to comprise Gram-negative, non-motile, non-spore-forming rods that produced yellow-pigmented colonies on R2A agar. The strain
Preparation and uses of photobiotin
Methods in Enzymology, 184, 588-600 (1990)
Rhodanobacter thiooxydans sp. nov., isolated from a biofilm on sulfur particles used in an autotrophic denitrification process
Lee CS, et al.
International Journal of Systematic and Evolutionary Microbiology, 57(8), 1775-1779 (2007)
Long Jin et al.
International journal of systematic and evolutionary microbiology, 57(Pt 8), 1876-1880 (2007-08-09)
Two novel bacteria, strains TR7-09(T) and P2-12-1, were isolated from samples of compost and river sediment, respectively. The strains comprised Gram-negative, motile, non-spore-forming rods, produced creamy white colonies on R2A agar, contained Q-8 as the predominant ubiquinone, contained iso-15 :
Kwang Kyu Kim et al.
International journal of systematic and evolutionary microbiology, 55(Pt 5), 2075-2079 (2005-09-17)
A novel xylan-degrading bacterium, S3-E(T), was isolated from the biofilm of a membrane bioreactor. The cells of this strain were Gram-positive, non-motile, non-spore-forming rods, produced primary branches and formed yellow colonies on nutrient agar. The strain had chemotaxonomic markers that

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