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Merck
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文件

A1752

Sigma-Aldrich

腺苷-5'-单磷酸 钠盐

from yeast, ≥99%

别名:

5′-腺苷酸, A-5′-P, AMP

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About This Item

经验公式（希尔记法）:
C10H14N5O7P · xNa+ · yH2O
CAS号:
149022-20-8
分子量:
347.22 (anhydrous free acid basis)
分類程式碼代碼:
41106305
eCl@ss:
32160414
PubChem物質ID:
24890583
NACRES:
NA.51

生物源

yeast

品質等級

300

化驗

≥99%

形狀

powder

溶解度

H2O: soluble

儲存溫度

−20°C

SMILES 字串

NC1=NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](O)[C@]((COP(O)(O)=O)([H])O3.C.

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應用

5′-单磷酸腺苷钠盐已用于：
  • 作为再激活培养基组分，再激活从附睾尾提取的小鼠精子，进行精子动力研究
  • 作为通过可逆交换实现放大信号(SABRE)方法的底物，建立生物相容性SABRE超极化
  • 作为小麦和黑曲霉Aspergillus niger植酸酶检测中的底物
  • 用于荧光素酶检测，监测S1S2细胞内的α-突触核蛋白低聚反应
  • 研究其对心脏骤停(CA)和复苏大鼠模型中治疗诱导性低温和神经损伤的影响

生化／生理作用

5′-单磷酸腺苷(5′-AMP)可用于增强肉和禽肉汤的鲜味。还可作为食品添加剂满足特别的营养需求。5′-AMP是AMP活化蛋白激酶(AMPK)这类蛋白激酶的激活剂。 AMP可协同肌苷单磷酸(IMP)介导骨骼肌肌动球蛋白分解为肌动蛋白和肌球蛋白。因此，这两种因子可能有参与肌肉解僵。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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D Grahame Hardie
Genes & development, 25(18), 1895-1908 (2011-09-23)
AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase
Alexey S Kiryutin et al.
Magnetic resonance in chemistry : MRC (2021-06-05)
Signal amplification by reversible exchange (SABRE) is a popular method for generating strong signal enhancements in nuclear magnetic resonance (NMR). In SABRE experiments, the source of polarization is provided by the nonthermal spin order of parahydrogen (pH2 , the H2
Daoying Wang et al.
Journal of the science of food and agriculture, 96(5), 1467-1473 (2015-05-09)
Adenosine 5'-monophosphate (AMP) is often used in meat and poultry soups as a flavor enhancer (flavor modifier), or as food additives for specific nutritional purposes. Our previous research as well as evidence from others showed that actomyosin could be dissociated
Akihiro Okitani et al.
Bioscience, biotechnology, and biochemistry, 72(8), 2005-2011 (2008-08-08)
We investigated to determine why heating of squid muscle at 60 degrees C induced the liberation of actin from myofibrils. When a mixture of a myofibrillar fraction and a low-molecular sarcoplasmic fraction prepared from squid muscle was heated at 60
The oxygen isotope composition of phosphate released from phytic acid by the activity of wheat and Aspergillus niger phytase
von Sperber C, et al.
Biogeosciences (Online), 12(13), 4175-4184 (2015)
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实验方案

5'-核苷酸酶的酶促检测

在测定5′-核苷酸酶活性时，本操作流程使用5′-单磷酸腺苷和一种显色试剂生成的标准曲线而对释放的磷的微摩尔数进行测定。

Enzymatic Assay of 5’-Nucleotidase

To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.

LC/MS Analysis of Nucleotides on SeQuant ZIC-cHILIC

HILIC separation is an alternative that permits sensitive MS detection and without the use of ion-pair reagents.

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