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Key Documents

53654

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR 600 antibody produced in goat

for STED application

别名:

Abberior® STAR 600-Anti-Rabbit IgG antibody produced in goat

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.45

生物源

goat

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

rabbit

存貨情形

available only in USA and Canada

濃度

~1 mg/mL

螢光

λex 604 nm; λem 623 nm in PBS, pH 7.4

儲存溫度

−20°C

一般說明

Abberior STAR 600 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 600 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. It can be effectively excited with excitation light between 550 - 610 nm. Abberior STAR 600 can substitute dyes like Atto 590, Atto 594 or Alexa Fluor 594. For STED microscopy, Abberior STAR 600 can be most efficiently used with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 600 performs exceptionally well in combination with Abberior STAR 635P, STAR 635 or STAR RED. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 604 (PBS pH 7.4), 604 (H2O), 601 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: : 95 000 (PBS pH 7.4), 95 000 (H2O), 100 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,25 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,25 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 623 (PBS pH 7.4), 623 (H2O), 620 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,85 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,2 (PBS pH 7.4)

適合性

Designed and tested for fluorescent super-resolution microscopy

分析報告

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

法律資訊

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Tobias Müller et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 13(8), 1986-2000 (2012-03-01)
For about a decade, superresolution fluorescence microscopy has been advancing steadily, maturing from the proof-of-principle stage to routine application. Of the various techniques, STED (stimulated emission depletion) microscopy was the first to break the diffraction barrier. Today, it is a

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