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生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
lyophilized
包含
≤0.1% sodium azide as preservative
物種活性
rat, mouse
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG
運輸包裝
ambient
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
mouse ... Calca(12310)
一般說明
兔多克隆抗CGRP可识别大鼠杏仁核和脊髓中的α-CGRP。它已被验证可用于免疫荧光，&可与冷冻、石蜡和自由浮动切片一起使用。
兔多克隆抗体以未稀释的血清形式提供。可识别α-CGRP蛋白。
可识别大鼠杏仁核和脊髓中的α-降钙素基因相关肽。
免疫原
大鼠α-降钙素基因相关肽（α-CGRP）
應用
自由浮动切片（参见应用参考文献）
冷冻切片（1:2000-1:4000，ABC检测）
免疫荧光（1:100-1:200，请参阅应用参考文献）
石蜡切片（请参阅应用参考文献；需要预先热处理；参见注释）
警告
毒性：标准处理（A）
重構
用100 µl无菌蒸馏H₂O复溶冻干抗体。注意复溶小瓶的全部内容物；在运输和处理过程中，冻干颗粒的部分可能已脱落，可能不在小瓶底部。溶解后，等分并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。用无菌PBS或Tris缓冲液稀释，稀释度不高于1:10。避免溶液反复冻融。
分析報告
阳性对照
大鼠杏仁核或脊髓
大鼠杏仁核或脊髓
其他說明
在大鼠杏仁核和脊髓中检查了抗体特异性。用10-5M大鼠α-CGRP预处理抗体可完全消除染色，用大鼠β-CGRP预吸收可部分消除染色。降钙素的预吸收不会导致免疫染色的损失。对于石蜡切片染色，将切片在40 mM枸橼酸钠缓冲液（pH 8.5）中加热至80°C。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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