MABS480

Anti-PTPN22 Antibody, clone 3B3.1

culture supernatant, clone 3B3.1, from mouse

• 别名: Tyrosine-protein phosphatase non-receptor type 22, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, Lymphoid phosphatase, LyP, PEST-domain phosphatase, PEP
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: 3B3.1, monoclonal
• 物種活性: human
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_057051
• UniProt登錄號: Q9Y2R2
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PTPN22(26191)

说明

一般說明

Tyrosine-protein phosphatase non-receptor type 22 (EC:3.1.3.48; UniProt Q9Y2R2; also known as Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, Lymphoid phosphatase, Lymphoid-specific protein tyrosine phosphatase, Lyp, PEP, PEST-domain phosphatase, Protein tyrosine phosphatase non-receptor type 8) is encoded by the PTPN22 (also known as LYP, LYP1, LYP2, PTPN8) gene (Gene ID 26191) in human. PTPN22/Lyp is expressed in all hematopoietic cells and belongs to the PEST group of nonreceptor classical class I protein tyrosine phosphatases (PTPs). Lyp is a negative regulator of TCR signal transduction by targeting phosphorylated Lck (Y394), Fyn (Y427), and Zap-70, as well as phospho-sites on TCRζ, CD3ε, Vav, and Vcp. Lyp utilizes its proline-rich region 1 to interact with the SH3 domain of C-terminal Src kinase (Csk), resulting in enhanced inhibitory tuning of Src family kinases and TCR signal transduction. TRAF3 is also shown to bind the proline-rich region 1 of Lyp in myeloid cells and impact its turnover. Additional binding partners of Lyp include the adaptor molecule Grb2, the E3 ligase c-Cbl.

特異性

Clone 3B3.1 is expected to react with spliced isoform 1 (LyP1), isoform 3, and isoform 4 (LyP3), but not isoform 2 (LyP2) or isoform 5. Reactivity toward isoform 6 (PTPN22.6) has not been tested.

免疫原

Epitope: C-terminal

GST-tagged recombinant protein corresponding to the C-terminal of human PTPN22.

應用

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-PTPN22 Antibody, clone 3B3.1 is validated for use in Western Blotting for the detection of PTPN22.

品質

Evaluated by Western Blotting in human burkitt′s lymphoma Ramos cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected PTPN22 in 10 µg of human burkitt′s lymphoma Ramos cell lysate.

標靶描述

~92 kDa observed. 91.7 kDa (isoform 1; Lyp1), 85.1 kDa (isoform 3), 88.6 kDa (isoform 4; Lyp3) calculated. Uncharacterized band(s) may appear in some lysates.

外觀

Unpurified

Mouse monoclonal IgG1κ in supernatant without preservatives.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2