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生物源
rat
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
18H2, monoclonal
物種活性
human, mouse
技術
western blot: suitable
同型
IgG2aκ
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
human ... APAF1(317)
mouse ... Apaf1(11783)
一般說明
Apoptotic protease-activating factor 1 (UniProt: O88879; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 11783) in murine species. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.
特異性
Clone 18H2 detects Apaf-1 in human and mouse cells. It targets an epitope within the first 97 amino acids from the N-terminal region containing the CARD domain.
免疫原
Epitope: N-terminus
Recombinant fragment corresponding to first 97 amino acids from the N-terminal region of murine Apaf-1.
應用
Anti-Apaf-1 Antibody, clone 18H2, Cat. No. MABC1187, is a rat monoclonal antibody that specifically detects Apaf-1. It has been tested for use in Western Blotting.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Scott, C.L., et. al. (2004). J Cell Biol. 164(1):89-96).
Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Marsden, V.S., et. al. (2006). Blood. 107(5):1872-7).
Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Marsden, V.S., et. al. (2006). Blood. 107(5):1872-7).
品質
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apaf-1 in 10 mg of SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apaf-1 in 10 mg of SH-SY5Y cell lysate.
標靶描述
~130 kDa observed;141 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外觀
Protein G purified
Format: Purified
Purified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
N V Srikanth Vallabani et al.
Mutagenesis, 34(3), 265-277 (2019-07-05)
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