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Merck
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主要文件

MABC1185

Sigma-Aldrich

Anti-ING3 Antibody, clone 2A2

clone 2A2, from mouse

别名:

Inhibitor of growth protein 3, p47ING3

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

2A2, monoclonal

种属反应性

mouse, human

技术

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG1κ

NCBI登记号

UniProt登记号

运输

ambient

靶向翻译后修饰

unmodified

基因信息

human ... ING3(54556)

一般描述

Inhibitor of growth protein 3 (UniProt: Q9NXR8; also known as ING3, p47ING3) is encoded by the ING3 (also known as HSPC301) gene (Gene ID 54556) in human. ING3 is a ~50 kDa protein that belongs to a family of proteins that contain PHD-finger domain and act as readers of the epigenetic code through specific recognition of trimethylated lysine 4 of histone H3. ING3 is a component of the NuA4 histone acetyltransferase (HAT) complex, which is involved in transcriptional activation of select genes, principally by acetylation of nucleosomal histones H4 and H2A. This complex is also shown to activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. ING3 is highly expressed in small intestine, bone marrow, epidermis, and in tissues where cells undergo rapid proliferation and renewal and its expression is shown to be higher in proliferating cells. Overexpression of ING3 has been shown to inhibit cell growth and induce apoptosis. ING3 is reported to be down-regulated in squamous cell carcinoma of the head and neck.

特异性

Clone 2A2 detected recombinant human ING3, but not INGs 1, 2, 4 or 5. Target band detection by Western blotting and immunocytochemistry. Staining by clone 2A2 was abolished using ING3 shRNA-transfected cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

免疫原

GST-tagged recombinant full-length human ING3.

应用

Detect ING3 using this mouse monoclonal Anti-ING3 Antibody, clone 2A2, Cat. No. MABC1185, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ING3 immunoreactivity in human small intestine tissue section.

Immunocytochemistry Analysis: A representative lot detected upregulated and downregulated ING3 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells transfected with ING3 and ING3 shRNA, respectively. Preabsorption with fixed ING3-overexpressing cells prevented subsequence immunocytochemical staining by clone 2A2 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Immunohistochemistry Analysis: A representative lot detected differential ING3 immunoreactivity among formalin-fixed paraffin-embedded human tissue sections, inluding hematopoietic tissues (bone marrow, lymphatic, thymus, and spleen) and epithelium of various tissue types (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Immunoprecipitation Analysis: A representative lot immunoprecipitated HA-tagged ING3 exogenously expressed in HEK293 cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Western Blotting Analysis: A representative lot detected endogenous ING3 in human HEK293 cells, a panel of murine tissues and cells (BALB-3T3 and NIH-3T3) as well as exogenously overexpressed ING3 in HEK293 cells, ING3 shRNA transfection abolished target band detection. Clone 2A2 detected bacterially rexpressed recombinant human ING3, but not INGs 1, 2, 4 or 5 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Western Blotting Analysis: A representative lot detected growth factor-induced ING3 expression in serum-starved HME human mammary fibroblasts immortalized with hTERT and RWPE-1 prostate epithelial cells immortalized with HPV18 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

质量

Evaluated by Immunohistochemistry in human ovarian cancer tissue.



Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ING3 immunoreactivity in human ovarian cancer tissue section.

目标描述

46.74 kDa calculated. ~50 kDa reported (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

外形

Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1


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