推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
1.D5, monoclonal
物種活性
human, rat
製造商／商標名
Chemicon®
技術
western blot: suitable
同型
IgG1
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... APP(351)
特異性
Reacts with the natural amyloid precursor protein A4(695).
免疫原
Epitope: A4(695)
Synthetic peptide Alz 90 [amino acids 511-608 of amyloid precursor protein pre A4(695)].
應用
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5 is validated for use in WB for the detection of Amyloid Precursor Protein.
Western blot: 10-20 μg/mL
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
外觀
Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
儲存和穩定性
Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Christine Villegas et al.
Human molecular genetics, 23(6), 1631-1643 (2013-11-10)
The amyloid-β precursor protein (APP), a type I transmembrane protein genetically associated with Alzheimer's disease, has a complex biology that includes proteolytic processing into potentially toxic fragments, extensive trafficking and multiple, yet poorly-defined functions. We recently proposed that a significant
Virgil Muresan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(11), 3565-3578 (2009-03-20)
The amyloid-beta (Abeta) precursor protein (APP), a transmembrane protein that undergoes proteolytic cleavage into defined fragments, has been implicated in axonal transport. The proposed role of APP as a vesicle receptor for the microtubule motor kinesin-1 has relevance for the
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