MAB2254

Anti-Tropomyosin Antibody, clone 15D12.2

clone 15D12.2, from mouse

• 别名: tropomyosin 1 (alpha), cardiomyopathy, hypertrophic 3, tropomyosin 1 alpha chain, HTM-alpha, Alpha-tropomyosin, chromosome 15 open reading frame 13, Tropomyosin-1, sarcomeric tropomyosin kappa, alpha tropomyosin
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 15D12.2, monoclonal
• 物種活性: human, mouse
• 物種活性（以同源性預測）: rat (based on 100% sequence homology)
• 技術: immunohistochemistry: suitable; western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_001029243
• UniProt登錄號: Q923Z2
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TPM1(7168)

说明

一般說明

Tropomyosins (Tms) are a family of >40 highly conserved protein isoforms resulting from alternative splicing of four different genes during development and that are expressed in numerous cell types. These proteins form coiled-coil heterodimers which bind to actin polymers along the α-helical grove. Tropomyosin’s function varies depending on cell type in which it is expressed. Within muscle, Tms play a role in the regulation of actin-myosin interplay for the purpose of mediating muscle contraction. In non-muscle cells, studies suggest a role in the stabilization of actin filaments, along with protecting actin filaments from the severing action of gelsolin and ADF/cofilin depolymerization. Tms also appear to have a role in the moderation of access to actin for other actin-binding proteins. Tropomyosin has an integral role in maintaining cardiovascular homeostasis. Down-regulation of Tms expression has been observed in tumor cells indicating a possible role for Tms as tumor suppressors.

特異性

The antibody recognizes the entire exon 9d of Tropomyosin.

免疫原

Epitope: Exon 9d

Linear peptide corresponding to the entire exon 9d of rat Tropomyosin.

應用

Detect Tropomyosin using this Anti-Tropomyosin Antibody, clone 15D12.2 validated for use in WB, IH.

Immunohistochemistry Analysis: 1:300 dilution from a representative lot detected Tropomyosin in human striated muscle tissue.

Research Category
Cell Structure

Metabolism

Research Sub Category
Cytoskeleton

Muscle Physiology

品質

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of the antibody detected Tropomyosin in 10 µg of mouse brain tissue lysate.

標靶描述

~ 30-40 kDa observed MWs. Tm6: 40 kDa; Tm1: 38 kDa; Tm2: 36 kDa; Tm3: 34 kDa; Tm5: 30 kDa

外觀

Protein G

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Mouse brain tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable