MAB1789

Anti-Migration Inhibitory Factor Related Proteins 8 and 14 Antibody, clone AHN-17

clone AHN-17, Chemicon^®, from mouse

• 别名: MRP-8 & 9, Myeloid-Related Protein-8 & 9, Calgranulin A & B, S100 Calcium-binding Protein A8 & A9, Leukocyte L1 cplx Light & Heavy Chain, Calprotectin
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: AHN-17, monoclonal
• 物種活性: human
• 製造商／商標名: Chemicon^®
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG1
• NCBI登錄號: NM_002964.3
• UniProt登錄號: P05109
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... S100A8(6279)

说明

一般說明

Protein S100-A8/9 (UniProt: P05109/ P06702; also known as MRP-8/MRP-14, Leukocyte L1 complex light chain/ Leukocyte L1 complex heavy chain, p8/p14, S100 calcium-binding protein A8/A9) are encoded by the S100A8/S100A9 (also known as MRP8/MRP14) genes (Gene ID: 6279/6280) in human. MRP-8 and MRP-14 are calcium- and zinc-binding protein that play a prominent role in the regulation of inflammatory processes and immune response. They are known to induce neutrophil chemotaxis and adhesion. They are predominantly found as calprotectin and perform a variety of intra- and extracellular functions. Their intracellular functions include facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes, and activation of the neutrophilic NADPH-oxidase. They are known to activate NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane. MRP-8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. Their extracellular functions include proinflammatory, antimicrobial, oxidant-scavenging and induction of apoptosis. MRP-14 is reported to induce neutrophil chemotaxis and adhesion and can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 pathways and can induce degranulation of neutrophils by a MAPK-dependent mechanism. MRP-8 and MRP-14 also acts as danger associated molecular pattern (DAMP) molecules and stimulate innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end (AGER) products. They also play an impotent role as oxidant scavengers and have a protective role in preventing exaggerated tissue damage by scavenging oxidants. (Ref.: Atallah, M., et al. (2012). PloS One. 7(2); e29333; Nakatani, Y., et al. (2005). Mediators Inflamm. 2005(5); 280-292; Ryckman, C., et al. (2003). J. Immunol. 170(6); 3233-3242).

特異性

Migration Inhibitory Factor Releasing Proteins -8 and -14

免疫原

Neutrophils

應用

Detect Migration Inhibitory Factor Related Proteins 8 & 14 using this Anti-Migration Inhibitory Factor Related Proteins 8 & 14 Antibody, clone AHN-17 validated for use in WB & IC.

Immunocytochemistry on ethanol fixed cells

Immunoblotting on un-reduced gels

Optimal dilutions must be determined by the end user.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

聯結

Replaces: CBL269

外觀

Format: Purified

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

儲存和穩定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable