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生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
A-Gel VC2, monoclonal
物種活性
human
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
同型
IgG1
適合性
not suitable for Western blot
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... MMP2(4313)
一般說明
MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer or the stromal cells. There is a cooperation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.
特異性
MAB13431 recognizes proteins of 72kDa and ~66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kDa collagenase IV or gelatinase A). CHEMICON MAB-13431 shows no cross-reaction with pro and active forms of other MMPs.
CELLULAR LOCALIZATION:
Cytoplasmic.
CELLULAR LOCALIZATION:
Cytoplasmic.
免疫原
Epitope: pro and active form
Human APMA-activated native 72kDa Gelatinase A.
應用
Anti-MMP-2 Antibody, pro & active form, clone A-Gel VC2 is an antibody against MMP-2 for use in ELISA, IH, IH(P).
Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 μg/mL 30 min at RT. Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.
ELISA (not suitable for coating, contains BSA) 1:200-1:2000
Not suitable for Western blotting
Optimal working dilution should be determined by the end user.
ELISA (not suitable for coating, contains BSA) 1:200-1:2000
Not suitable for Western blotting
Optimal working dilution should be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
聯結
Replaces: 04-1048
外觀
10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide
Format: Purified
儲存和穩定性
Antibody is stable for 12 months when stored at 2-8ºC
分析報告
Control
POSTIVE CONTROL:
Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.
POSTIVE CONTROL:
Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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A B Frey et al.
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