推荐产品
生物源
mouse
品質等級
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
C11, monoclonal
物種活性
human
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunohistochemistry: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... COL1A1(1277)
特異性
Recognizes both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and II in ELISA, RIA or immunoblotting.
應用
Detect Collagen Type I using this Anti-Collagen Type I Antibody, clone C11 validated for use in ELISA, IH.
Immunohistochemical staining of 10 μm thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is not recommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.
ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.
Optimal working dilutions must be determined by the end user.
ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.
Optimal working dilutions must be determined by the end user.
外觀
Immunoglobulin Purified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose. . Lyophilized, reconstitute with 1 mL of distilled water.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 2
Patricia Khashayar et al.
3 Biotech, 7(5), 312-312 (2017-09-29)
This article explains a step-wise protocol to develop an electrochemical sensor to quantify serum levels of C-telopeptide (CTX) crosslinks also known as crosslaps in a matter of minutes and with high level of accuracy. The technique needs only one-step (incubation)
Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence.
Zoumi, A; Yeh, A; Tromberg, BJ
Proceedings of the National Academy of Sciences of the USA null
Human tendon stem cells better maintain their stemness in hypoxic culture conditions.
Zhang, J; Wang, JH
Testing null
Jose M Gonzalez et al.
Scientific reports, 6, 21315-21315 (2016-02-18)
The contractile trabecular meshwork (TM) modulates aqueous humor outflow resistance and intraocular pressure. The primary goal was to visualize and quantify human TM contractile state by analyzing actin polymerization (F-actin) by 2-photon excitation fluorescence imaging (TPEF) in situ. A secondary
Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.
Ding, Z; Huang, H
BMC Musculoskelet. Disord. null
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