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一般說明
Substances with low molecular weights are generally not immunogenic and require the aid of carrier proteins to stimulate an immune response. Carrier proteins have relatively high molecular weights and carry many epitopes. An immune response to a hapten-carrier conjugate results in antibodies that are specific for both the hapten and the carrier. Hemocyanin is a high molecular weight respiratory metalloprotein found in the hemolymph of many mollusks and crustaceans. Molluscan hemocyanin is based on an approximately 400 kDa polypeptide subunit that is folded into a chain of multiple, immunologically distinct domains, each with a binuclear copper site. Keyhole Limpet Hemocyanin (KLH), the hemocyanin from the giant keyhole limpet, Megathura crenulata, is the most widely used carrier protein. It is an extremely large protein (6 - 7.5 million daltons) that can exist as a di-decamer, multi-decamers, or flexible tubules of varying length. It is easily dissociated into subunits and the dissociation-association reaction depends on protein concentration, ionic strength, and pH. At pH 6.5, KLH exists in a fully associated state without any evidence of dissociation products. In contrast, at pH 8.5 in borate saline the molecule is completely dissociated.
特異性
Clone 3B1 detects keyhole limpet hemocyannin (KLH) and KLH conjugated to peptide or protein.
免疫原
Purified Keyhole limpet hemocyanin from Megathura crenulata
應用
Anti-KLH, clone 3B1, Cat. No. MAB131871, is a mouse monoclonal antibody that detects Keyhole limpet hemocyanin and has been tested for use in Western Blotting.
Research Category
Inflammation & Immunology
Inflammation & Immunology
品質
Evaluated by Western Blotting with Keyhole Limpet Hemocyanin (KLH).
Western Blotting Analysis: A 1:500 dilution of this antibody detected Keyhole Limpet Hemocyanin.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Keyhole Limpet Hemocyanin.
標靶描述
~375 kDa observed; Calculated ~ 6 to 7.5 MDa (undissociated). Uncharacterized bands may be observed in some lysate(s).
外觀
Format: Purified
Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
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