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LIF2010

Sigma-Aldrich

小鼠白血病抑制因子

10 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

别名:

LIF, Differentiation-stimulating Factor, D Factor, Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.75

生物源

mouse

品質等級

300

化驗

>95% (HPLC and SDS-PAGE)

形狀

liquid

比活性

≥1 x 10(E8) U/mg

製造商／商標名

Chemicon®

濃度

10 μg/mL

技術

cell culture | stem cell: suitable

雜質

<0.1 ng/mg Endotoxin (of LIF)

輸入

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)

NCBI登錄號

NM_008501

UniProt登錄號

P09056

運輸包裝

wet ice

應用

推荐的QC方案

M1生物测定

1.使用体外半固体琼脂培养物进行M1生物测定，该培养物在1 mL体积的DME（含20%FCS的0.3%琼脂）中含有约100个细胞。

2.加入100 μL样品或mLIF（等渗盐水中5%FCS中的10（E4）单位/mL），一式两份两倍系列稀释至35 mm培养皿。

3.将100μ L 5%FCS的等渗盐水加入到两个对照载玻片上。

4.在37°C、含10%CO2的完全湿润空气中培养7天。

5.对显示分化的菌落数进行评分（注：50单位定义为导致50%菌落分化的活性量）。

有关其他信息，请访问www.esgro-lif.com

LIF由CHEMICON International，Inc.生产，受美国专利号5,187,077、5,427,925、5,443,825、5,750,654 和 6,261,548，欧洲专利号 0285 448 和相关的外国专利的保护，并无法再销售。

單位定義

比活度：50 个单位定义为在 1 mL 琼脂培养物中诱导 50％ M1 克隆分化所需的小鼠 LIF 量。

分析報告

无菌和微质体测试均为阴性。
比活度：小鼠 LIF 的活性取决于诱导小鼠 M1 髓样白血病细胞分化的能力。在该测定法中，小鼠LIF的最低可检测浓度为 0.5 ng/mL

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Kay Jüngling et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 17(14), 2100-2102 (2003-09-23)
The pluripotency and high proliferative capacity of embryonic stem (ES) cells (1-3) makes them an attractive source of different cell types for biomedical research and cell replacement therapies. A major prerequisite for these applications is the availability of a homogeneous
Eunhye Kim et al.
PloS one, 11(7), e0160289-e0160289 (2016-07-30)
Pigs provide outstanding models of human genetic diseases due to their striking similarities with human anatomy, physiology and genetics. Although transgenic pigs have been produced using genetically modified somatic cells and nuclear transfer (SCNT), the cloning efficiency was extremely low.
Hsiao-Wen Lin et al.
Journal of neuroinflammation, 6, 7-7 (2009-03-10)
Ciliary neurotrophic factor (CNTF) has been regarded as a potent trophic factor for motor neurons. However, recent studies have shown that CNTF exerts effects on glial cells as well as neurons. For instance, CNTF stimulates astrocytes to secrete FGF-2 and
Ting Ting Li et al.
Journal of veterinary science, 21(1), e13-e13 (2020-01-16)
Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement)
Emma C Walker et al.
The Journal of biological chemistry, 291(41), 21703-21716 (2016-08-20)
Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are IL-6 family members with a wide range of biological functions. Human OSM (hOSM) and murine LIF (mLIF) act in mouse cells via a LIF receptor (LIFR)-glycoprotein 130 (gp130) heterodimer. In contrast
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