ABN1687

Anti-TBR-2

from rabbit

• 别名: Eomesodermin homolog, T-box brain protein 2, T-brain-2
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: rabbit
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 种属反应性: mouse
• 种属反应性（根据同源性预测）: rat (based on 100% sequence homology)
• 包装: antibody small pack of 25 μg
• 技术: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同位素/亚型: IgG
• NCBI登记号: NP_034266
• UniProt登记号: O54839
• 靶向翻译后修饰: unmodified
• 基因信息: mouse ... Eomes(13813)

说明

一般描述

Eomesodermin homolog (UniProt: O54839; also known as T-box brain protein 2, T-brain-2, TBR-2) is encoded by the Eomes (also known as Tbr2) gene (Gene ID: 13813) in murine species. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. TBR-2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is also shown to be involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region is localized to amino acids 278-458. Conditional inactivation of TBR-2 during early brain development is known to cause microcephaly and severe behavior deficits. Two isoforms of TBR-2 have been described that are produced by alternative splicing. Isoform 2 lacks amino acids 463-481. (Ref.: Bulfone, A., et al. (1999). Mech. Dev. 84 (1-2); 133-138).

特异性

This rabbit polyclonal antibody detects T-box brain protein 2 (TBR-2) in murine species. It targets an epitope with in 20 amino acids from the C-terminal region.

免疫原

Conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of murine TBR-2.

Epitope: C-terminus

应用

Anti-TBR-2, Cat. No. ABN1687, is a highly specific rabbit polyclonal antibody that targets Eomesodermin homolog (Tbr2) and has been tested in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected TBR-2 in Immunofluorescence applications (Nelson, B.R., et. al. (2013). J Neurosci. 33(21):9122-39; Hodge, R.D., et. al. (2013). J Neurosci. 33(9):4165-80).

Immunohistochemistry Analysis: A representative lot detected TBR-2 in Immunohistochemistry applications (Yoon, K.J., et. al. (2008). Neuron. 58(4):519-31).

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected TBR-2 in mouse hippocampus, embryonic mouse brain, and embryonic mouse gut tissue sections.

Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in mouse E13-14 brain.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TBR-2 in mouse E13-14 brain.

目标描述

~63 kDa observed; 74.80 kDa calculated.

外形

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.