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Merck
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文件

ABE457

Sigma-Aldrich

抗-c-Fos抗体

from rabbit, purified by affinity chromatography

别名:

Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human, rat

物種活性(以同源性預測)

sheep (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology)

技術

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... FOS(2353)

一般說明

c-Fos是也被鉴定为原癌基因的立即早期基因(IEG)转录因子家族的成员(其他成员包括Jun和Egr-1)。c-Fos和其他IEG的基础表达通常较低,但响应通常由外部刺激(例如应激或神经元激活)引起的细胞活性变化而相对快速且通常急剧增加(mRNA为15-30分钟;蛋白为30-60分钟)。因此,这些IEG用于指示比活驱动的细胞活化,包括鉴别由各种生长因子、神经活性药物激活的神经元以及行为或生理状态的相关变化。

特異性

该抗体识别c-Fos的N末端。

免疫原

对应于人c-Fos N末端的KLH偶联的线性肽。

應用

免疫组化分析: 代表性批次的1:1,000稀释液在大鼠脑桥和大鼠小脑组织中检测到c-Fos。

免疫荧光分析: 代表性批次在大鼠脑桥和大鼠小脑组织中检测到c-Fos。
抗c-Fos抗体是一种用于检测c-Fos的兔多克隆抗体,也称为原癌基因c-Fos,细胞癌基因fos&已在WB &IHC中得到验证。

品質

通过蛋白质印迹在PMA(TPA)处理的HeLa细胞裂解物中的进行了评估。

蛋白质印迹分析:0.5 µg/mL该抗体在10 µg PMA(TPA)处理的HeLa细胞裂解物中检测到c-Fos。

標靶描述

观察值〜60/56 kDa。 该蛋白具有通过53和68kDa范围内的选择性剪接产生的多种亚型(Jurado, J., et al. (2007).BMC Molecular Biology.8:83-96.)

分析報告

对照
PMA(TPA)处理的HeLa细胞裂解物

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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