推荐产品
生物源
rabbit
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
human
製造商/商標名
Chemicon®
技術
western blot: suitable
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
phosphorylation (pSer409)
基因資訊
human ... MAPT(4137)
一般說明
Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer′s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Serine 409 is phosphorylated by PKA in vitro and has shown to be phosphorylated in AD brain.
特異性
Tau phosphoSerine 409. The antibody recognizes Tau pSerine 409 in samples of recombinant human Tau treated with PKA for 45 minutes. The reactivity of the antibody is blocked with the pSerine 409 peptide but not the non-phosphopeptide or a generic phosphoSerine-containing peptide.
免疫原
Synthetic peptide of amino acids surrounding the phosphoSerine 409 site of human Tau.
應用
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Tau phospho Serine 409 Antibody is validated for use in WB for the detection of Tau phospho Serine 409.
Western blot: 1:1,000. Suggested blocking buffer is 5% BSA-TBST for one hour at room temperature. Suggested antibody dilution buffer is 1% BSA-TBST. Suggested antibody incubation time is 2 hours at room temperature.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
儲存和穩定性
Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
Peripheral B cells may serve as a reservoir for persistent hepatitis C virus infection.
Journal of Innate Immunity null
Journal of clinical medicine, 8(10) (2019-10-24)
Based on the protein kinase A (PKA)/GSK3β interaction protein (GSKIP)/glycogen synthase kinase 3β (GSK3β) axis, we hypothesized that these might play a role in Tau phosphorylation. Here, we report that the phosphorylation of Tau Ser409 in SHSY5Y cells was increased
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