推荐产品
生物源
rabbit
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
mouse, rat
製造商/商標名
Chemicon®
技術
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... SCN1A(6323)
特異性
识别VGSC的I型α亚基。不与迄今为止检测的任何其他钠通道抗原发生交叉反应。
物种反应性: 由于序列同源性,预计该抗体也与人反应。其他物种尚未经过测试。
物种反应性: 由于序列同源性,预计该抗体也与人反应。其他物种尚未经过测试。
免疫原
来自大鼠I型电压门控钠通道(VGSC)的α亚基465-481的纯化肽(登录号P04774)。
應用
将要接收全长蛋白的全部程序应在4C下进行,并应使用以下蛋白酶抑制剂混合物:胃蛋白酶抑制剂A(1 μg/mL),亮抑蛋白酶肽(1 μg/mL),抑肽酶(1 μg/mL),Pefabloc SC(0.2 mM),苯甲脒(0.1 mg/mL)和钙蛋白酶抑制剂I和II(各8 μg/mL)。
蛋白质印迹:在大鼠脑膜上使用ECL为L1:200。
大鼠脑固定冰冻切片和小鼠心脏组织上的免疫组化。
应使用载体蛋白(例如BSA(1-3%))进行稀释。
最佳工作稀释度必须由最终用户确定。
蛋白质印迹:在大鼠脑膜上使用ECL为L1:200。
大鼠脑固定冰冻切片和小鼠心脏组织上的免疫组化。
应使用载体蛋白(例如BSA(1-3%))进行稀释。
最佳工作稀释度必须由最终用户确定。
该抗钠通道抗体,电压门控,脑I型经验证可用于IH,WB检测钠通道。
其他說明
浓度:请参考批次特异性浓缩物的分析证书。
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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