推荐产品
生物源
rabbit
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human, mouse
製造商／商標名
Chemicon®
技術
western blot: suitable
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... APAF1(317)
特異性
Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and was designated Apaf1 for apoptosis protease-activating factor 1(Zou et al. 1997; Cecconi et al. 1998). Apaf1 binds to cytochrome C (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 which is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Li et al. 1997). Apaf1 can also associate with caspase-4 and caspase-8 (Hu et al. 1998). Apaf1 is ubiquitously expressed in human tissues (Zou et al. 1997).
免疫原
Epitope: N-terminus
Polyclonal antibody raised against a peptide corresponding to amino acids 12 to 28 of human Apaf1 (Zou et al. 1997) The sequences of the immunogenic peptide are identical between human and mouse (Zou et al. 1997; Cecconi et al. 1998).
應用
Anti-Apaf-1 Antibody, N-terminus is an antibody against Apaf-1 for use in WB.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western blot: 1:1000 to 1:4000
Whole cell lysate from HeLa cells can be used as a positive control and a 130 kDa band should be detected. Optimal working dilutions must be determined by end user.
Whole cell lysate from HeLa cells can be used as a positive control and a 130 kDa band should be detected. Optimal working dilutions must be determined by end user.
聯結
Replaces: 04-579
外觀
Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Format: Purified
儲存和穩定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Carla L O'Connor et al.
Biochimica et biophysica acta, 1783(10), 1903-1913 (2008-07-02)
X-linked-inhibitor-of-apoptosis-protein (XIAP) is the most potent intracellular inhibitor of caspases-9, -3 and -7. While highly elevated XIAP levels reduce the apoptotic response to various stimuli, the potency of physiological XIAP expression to control caspase activation and the consequences of XIAP
Mary E Lucas et al.
The Journal of biological chemistry, 284(22), 14698-14709 (2009-03-28)
Epigenetic silencing involving the aberrant DNA methylation of promoter-associated CpG islands is one mechanism leading to the inactivation of tumor suppressor genes in human cancers. However, the molecular mechanisms underlying this event remains poorly understood. TMS1/ASC is a novel proapoptotic
Marco Corvaro et al.
Cancer biology & therapy, 6(2), 209-217 (2007-01-17)
The apoptosome is a multiprotein complex mediating the mitochondrial pathway of cell death. Its importance during development has been clearly demonstrated by knocking out key genes in mouse. APAF1 is the core protein of the apoptosome and its dosage is
Hui-Chen Chen et al.
Nature cell biology, 17(10), 1270-1281 (2015-09-08)
Multidomain pro-apoptotic BAX and BAK, once activated, permeabilize mitochondria to trigger apoptosis, whereas anti-apoptotic BCL-2 members preserve mitochondrial integrity. The BH3-only molecules (BH3s) promote apoptosis by either activating BAX-BAK or inactivating anti-apoptotic members. Here, we present biochemical and genetic evidence
Sheila Jeudy et al.
PloS one, 6(8), e22369-e22369 (2011-08-19)
Laminin α2 (LAMA2)-deficient congenital muscular dystrophy is a severe, early-onset disease caused by abnormal levels of laminin 211 in the basal lamina leading to muscle weakness, transient inflammation, muscle degeneration and impaired mobility. In a Lama2-deficient mouse model for this
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