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Merck
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主要文件

524715

Sigma-Aldrich

Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda

别名:

PDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.77

表单

liquid

质量水平

比活

≥50,000 units/mL

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

运输

wet ice

储存温度

−70°C

一般描述

Recombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583).
Recombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). For enzymatic studies a dilution of 1:1000 recommended. For use as a immunoblot standard 2 to 3 µl per lane is recommended. In immunoblotting, gives band at ~92 kDa.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.

外形

In sodium phosphate buffer, 20% glycerol, pH 7.2.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 1


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