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技術
protein clean-up: suitable
應用
sample preparation
一般說明
The Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. The Med column is suitable for removing detergents from a 1.0 mL solution of 1.5% Triton-X100 detergents (or an equivalent of 15 μL Triton-X100 or 15 mg detergents), provided that both the columns and proteins solutions are prepared in water.
應用
PROTOCOL:
1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.
2. Buffer Equilibration: Detergent-OUT columns are supplied in deionized water. A better result is obtained when the column and protein solution are in water or phosphate buffer. Columns may be equilibrated with other types of buffer. Note: Do not equilibrate the columns with Tris buffer and other such buffers. Apply about 1.0 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.
3. Place the column in a 15mL centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.
4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes..
5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.
2. Buffer Equilibration: Detergent-OUT columns are supplied in deionized water. A better result is obtained when the column and protein solution are in water or phosphate buffer. Columns may be equilibrated with other types of buffer. Note: Do not equilibrate the columns with Tris buffer and other such buffers. Apply about 1.0 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.
3. Place the column in a 15mL centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.
4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes..
5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
包裝
10 columns
成分
Detergent-OUT Columns-DTG-100X 10 Med
儲存和穩定性
Store at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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