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應用
sample preparation
一般說明
The Chemicon Protein-Concentrate kit uses a proprietary reagent Universal Protein Precipitation Agent (UPPA). Protein solutions as dilute as 1 ng/mL can be quantitatively concentrated into a small volume. Protein precipitation is not effected by the presence of detergents, chaotropic, or other common laboratory agents. After precipitation, the precipitate is suspended in a small volume of Precipitate Suspension Buffer supplied with each kit. If the protocol is followed correctly, the recovery is generally 100%. There are enough reagents for concentrating up to 30 mL of dilute protein solution.
應用
Suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays and other applications.
This kit is not suitable for those proteins which may lose some of their biological activities when precipitated.
PROTOCOL: ImportantPerform the entire procedure in the cold (ice bucket) unless specified otherwise. Concentration should be performed in a centrifuge tube. For small volumes, use microfuge tubes. Always position microfuge tubes in the centrifuge at the same orientation, i.e. cap-hinge facing out-ward, this will allow the pellet to remain glued to the same side of the tube during repeated centrifugations and minimize the loss of protein pellets. See product insert for protocol.
This kit is not suitable for those proteins which may lose some of their biological activities when precipitated.
PROTOCOL: ImportantPerform the entire procedure in the cold (ice bucket) unless specified otherwise. Concentration should be performed in a centrifuge tube. For small volumes, use microfuge tubes. Always position microfuge tubes in the centrifuge at the same orientation, i.e. cap-hinge facing out-ward, this will allow the pellet to remain glued to the same side of the tube during repeated centrifugations and minimize the loss of protein pellets. See product insert for protocol.
包裝
1 kit
成分
UPPA-I 100 mL UPPA-II 100 mL OrgoSol 2 x 50 mL UPC-SEED 2 x 0.30 mL Precipitate Buffer I 2 x 2.0 mL Precipitate Buffer II 2 x 0.5 mL UCP™-Wash 2 x 2.0 mL
儲存和穩定性
Store at room temperature for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
法律資訊
UCP is a trademark of Intelligent Material Solutions, Inc.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Danger
危險分類
Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
標靶器官
Central nervous system, Respiratory system
儲存類別代碼
3 - Flammable liquids
閃點（°F）
-2.2 °F
閃點（°C）
-19 °C
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