推荐产品
product name
Purospher® STAR Phenyl (5 µm), L × I.D. 250 mm x 4.6 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use
材料
stainless steel column
品質等級
agency
suitable for USP L11
產品線
Purospher®
特點
endcapped: no
包裝
1 ea of
標籤範圍
12.5% Carbon loading
參數
250 bar pressure
65 °C max. temp.
技術
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
柱長度 × 內徑
25 cm × 4.6 mm
表面積
330 m2/g
基質
fully porous particle
基質活性組
Phenyl bonding phase
粒徑
5 μm
孔徑
120 Å pore size
工作pH值範圍
1.5-10.5
分離技術
reversed phase
儲存溫度
no temp limit
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相关类别
一般說明
Purospher® STAR Phenyl, 5 µm UHPLC columns are the best alternative to RP-8 or RP-18 columns for the efficient separation of aromatic compounds, and compounds containing aromatic groups. Purospher® STAR Phenyl can retain analytes via several different mechanisms, including ο-ο interactions between the overlap of the delocalized electrons on the analyte and the stationary phase phenyl group, and via partitioning between the mobile phase and the hydrophobic aryl-alkyl phase. Our Purospher® STAR Phenyl columns are based on high purity silica particles, which provide symmetrical peaks for basic compounds, as well as high stability and excellent reproducibility. Furthermore, hydrophobic compounds elute much faster with Purospher® STAR Phenyl columns than with C-18 columns.Purospher® STAR Phenyl, 5 µm LiChroCART® 250-4.6 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001. LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.For higher pressure stability requirements Purospher® STAR Phenyl, 5 µm Hibar® RT 250-4.6 HPLC columns are available part number 1.51918.0001. The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for HPLC high-resolution separation of complex samples.Item 1.51921.1003 is a Validation kit containing 3 Purospher® STAR Phenyl, 5 µm LiChroCART® 250-4.6 HPLC columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide
應用
Intended to be used in the separation of antibiotics,aromatic amines, flavonoids, ketones,nucleosides, PAHs, peptides, preservatives, steroids, metabolized steroids, and sweeteners.
特點和優勢
- Ultra-pure silica (99.999%) for excellent peak symmetry
- High separation efficiency
- Enhanced selectivity for aromatic compounds
- Suitable for use with highly aqueous mobile phases
- Good suitability for LC-MS applications
法律資訊
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
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