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生物源
human
品質等級
化驗
~95% (SDS-PAGE)
形狀
liquid
儲存期限
1 yr
分子量
Mw 72 kDa
製造商／商標名
Upstate®
濃度
0.5 mg/mL
技術
activity assay: suitable
溶解度
water: soluble
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
基因資訊
human ... TP53(120407068)
一般說明
Research area: Apoptosis
N-terminal GST-tagged, recombinant full length human P53 protein, expressed in E. coli. Purified using glutathione agarose chromatography. Product Source: Full-length human p53 protein, expressed in e. coli. p53 protein is encoded by the tumor suppressor gene TP53 gene mapped on chromosome 17.
N-terminal GST-tagged, recombinant full length human P53 protein, expressed in E. coli. Purified using glutathione agarose chromatography. Product Source: Full-length human p53 protein, expressed in e. coli. p53 protein is encoded by the tumor suppressor gene TP53 gene mapped on chromosome 17.
生化／生理作用
In the cell, the p53 protein binds to DNA, leading to the activation of another gene that produces the p21 protein. This protein interacts with a cell division-stimulating protein (cdk2), preventing the cell from progressing to the next stage of cell division. Mutation in p53 protein leads to uncontrolled cell division resulting in tumor formation. The p53 protein is suggested to impede the phenotypic and genomic changes linked to cancer development through its intricate interaction with various signaling pathways including cell division, genomic stability maintenance, apoptosis, autophagy, immune response, and tumor microenvironment regulation.
品質
Routinely evaluated by SDS-PAGE and Coomassie Stain
外觀
Glutathione agarose chromatography
Recombinant protein in buffer containing 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 5mM glutathione, 0.5 mM EDTA, 0.5 mM DTT, 1 mM PMSF containing 20% glycerol.
儲存和穩定性
1 year at -20°C
其他說明
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
The p53 tumor suppressor protein
Bethesda MD
Genes & diseases (1998)
p53 signaling in cancer progression and therapy
Marei HE, et al.
Cancer Cell International, 21 (2021)
María Pedrero et al.
Biosensors, 6(4) (2016-11-24)
An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs
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