14-276-M
Akt1/PKBα Protein, active, 15 µg
Active, recombinant full-length human Akt1, containing N-terminal His6 tag, for use in IP-Kinase Assays & Kinase Assays.
别名:
Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha
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所有图片(2)
About This Item
分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.26
推荐产品
生物源
human
品質等級
重組細胞
expressed in baculovirus infected Sf21 cells
形狀
liquid
儲存期限
1 yr
分子量
Mw 59.9 kDa
製造商／商標名
Upstate®
技術
activity assay: suitable (IP-kinase)
activity assay: suitable (kinase)
溶解度
soluble
UniProt登錄號
儲存溫度
−70°C
基因資訊
human ... AKT1(207)
一般說明
Research area: CELL SIGNALING
Product Source: Expressed by baculovirus in Sf21 insect cells
Recombinant full-length human Akt1, containing N-terminal His6-tag.
Product Source: Expressed by baculovirus in Sf21 insect cells
Recombinant full-length human Akt1, containing N-terminal His6-tag.
生化／生理作用
Protein Target: Akt1/PKBα
Target Sub-Family: AGC
Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.
Target Sub-Family: AGC
Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.
包裝
Also available in 250μg size, please inquire for pricing and availability.
品質
Routinely evaluated by phosphorylation of Crosstide. Please refer to the Certificate of Analysis for more information.
外觀
Ni2+/NTA and glutathione agarose
Supplied in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.07% 2-mercaptoethanol, 0.02% Brij 35, 270mM sucrose. Frozen solution.
儲存和穩定性
1 year at -70°C
其他說明
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Danger
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 2
Insulin Receptor Signaling
Laychock SG
Encyclopedia of Hormones, 368-380 (2003)
AKT/PKB signaling: navigating downstream
Manning BD, et al.
Cell, 129(7), 1261-1274 (2007)
Structural Biology and Anticancer Drug Design
Pathuri P, et al.
Journal of Separation Science, 2, 121-141 (2014)
Madhavi J Rane et al.
The Journal of biological chemistry, 278(30), 27828-27835 (2003-05-13)
Activation of the serine-threonine kinase Akt by cytokines, chemokines, and bacterial products delays constitutive neutrophil apoptosis, resulting in a prolonged inflammatory response. We showed previously that Akt exists in a signaling complex with p38 MAPK, MAPK-activated protein kinase-2 (MAPKAPK-2), and
A common phosphate binding site explains the unique substrate specificity of GSK3 and its inactivation by phosphorylation
Frame, S., et al
Molecular Cell, 7, 1321-1327 (2001)
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