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Merck
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主要文件

1.32647

Sigma-Aldrich

Alcian blue staining kit, pH 1.0

for the detection of strongly sulfated mucins for microscopy

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About This Item

品質等級

IVD

for in vitro diagnostic use

pH值

1.0

應用

clinical testing
diagnostic assay manufacturing
hematology
histology

儲存溫度

15-25°C

一般說明

The ready-to-use Alcian blue stainig kit, pH 1.0 - for the detection of strongly sulfated mucins - for microscopy, can be used for this staining in histological tissue. Alcian blue pH 1.0 is used to stain strongly sulfated mucins in human-histological specimen materials, for example histological sections of e. g. colon or bronchus. The dye Alcian blue 8 GX (Product number 1.01647) is a water-soluble phthalocyanine dye. Acidic mucins have a negative charge and bind the positively charged Alcian blue 8 GX staining dye.
A solution of hydrochloric acid and Alcian blue is used for the staining procedure. At a strongly acidic pH of 1.0, this solution suppresses the dissociation of the carboxyl groups and selectively stains only the strongly sulfated mucins and polyanions. Counterstaining takes place with a nuclear fast red/aluminium sulfate solution. The undifferentiated method (Alcian blue 8 GX solution, pH 2.5) enables only the detection of acidic glycoproteins, e. g. mucins, without distinguishing between carboxyl and sulfate groups.
The package is sufficient for approx. 2500 applications. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.

應用

For the detection of strongly sulfated mucins.

分析報告

Nuclei: red
Strongly sulfated mucins: light blue

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Met. Corr. 1

儲存類別代碼

8A - Combustible, corrosive hazardous materials

水污染物質分類(WGK)

WGK 2


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