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Merck
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文件

126575

Sigma-Aldrich

白蛋白，牛血清，组分V，不含脂肪酸

别名:

白蛋白，牛血清，组分V，不含脂肪酸

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About This Item

CAS号:
9048-46-8
分類程式碼代碼:
12352202
NACRES:
NA.25

化驗

≥95% (cellulose acetate electrophoresis)

形狀

powder

製造商／商標名

Calbiochem®

儲存條件

OK to freeze

雜質

≤0.02% fatty acids
≤3% ash
≤5% moisture

顏色

white to light yellow
light yellow to light brown

運輸包裝

ambient

儲存溫度

2-8°C

相关类别

一般說明

牛血清白蛋白（BSA、组分V、不含脂肪酸）适用于免疫诊断分析。它设计用于血清学检测、放射免疫测定和激素反应研究。它也适用于结合和运输研究。BSA采用冷乙醇提取法（Cohn法）制备。pH值：6.8-7.4。
牛血清白蛋白（BSA）是一种非糖基化球状α-螺旋蛋白，属于血清白蛋白家族。它由三个结构域组成，每个结构域有两个子结构域，并且具有17个二硫键。

應用

牛血清白蛋白（组分V、不含脂肪酸）已用作：
  • 一种组分以用于各种细胞免疫电子显微镜（IEM）的阻断缓冲液
  • 线粒体隔离缓冲液（MIB）和线粒体测定液（MAS），以捕获脂肪组织均质化过程中释放的游离脂肪酸
  • 一种阻断缓冲液，以对食管上皮（EE）进行免疫荧光全旦染色

生化／生理作用

牛血清白蛋白（BSA）是细胞培养基中最重要的成分，有助于胚胎干细胞（hESC）的分化。它有助于药物、激素和脂肪酸的运输。BSA也被用作酶联免疫吸附试验（ELISA）的阻断剂。

警告

毒性：标准处理（A）

準備報告

可能需要长达24小时才能完全溶解。

重構

在复溶后存放于冰箱（4°C）。可能添加0.1% NaN₃作为防腐剂。储备溶液在4°C下可稳定保存3个月。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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M Prats
European journal of biochemistry, 75(2), 619-625 (1977-05-16)
The L-(+)-Lactate:cytochrome c oxidoreductase or cytochrome b2 from the yeasts Saccharomyces cerevisiae and Hansenula anomala were partially hydrolysed in various concentrations of trypsin. Conditions were found which allowed the isolation from the Hansenula enzyme of a 140 000 +/- 10
Andrew W Ferree et al.
Autophagy, 9(11), 1887-1896 (2013-10-24)
To study mitochondrial protein age dynamics, we targeted a time-sensitive fluorescent protein, MitoTimer, to the mitochondrial matrix. Mitochondrial age was revealed by the integrated portions of young (green) and old (red) MitoTimer protein. Mitochondrial protein age was dependent on turnover
Mohyeddine Omrane et al.
iScience, 13, 138-153 (2019-03-05)
Septins are GTP-binding proteins involved in several membrane remodeling mechanisms. They associate with membranes, presumably using a polybasic domain (PB1) that interacts with phosphoinositides (PIs). Membrane-bound septins assemble into microscopic structures that regulate membrane shape. How septins interact with PIs
Nick Osborne et al.
Current biology : CB, 18(23), 1882-1888 (2008-12-09)
The bioactive lipid sphingosine 1-phosphate (S1P) and its G protein-coupled receptors play critical roles in cardiovascular, immunological, and neural development and function. Despite its importance, many questions remain about S1P signaling, including how S1P, which is synthesized intracellularly, is released
Soyeon Park et al.
Experimental & molecular medicine, 50(8), 1-14 (2018-08-05)
Increased fatty acid (FA) is often observed in highly proliferative tumors. FAs have been shown to modulate the secretion of proteins from tumor cells, contributing to tumor survival. However, the secreted factors affected by FA have not been systematically explored.
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