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Merck
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文件

1.11350

Millipore

Bactident™ Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

别名:

4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.84

品質等級

100

描述

Bactident™ E. coli 1.13303

形狀

liquid (ready-to-use)

儲存期限

210 days

特點

ready-to-use

成份

(n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)

包裝

pkg of (1 x 30 ml, Plastic bottle)

技術

microbe id | specific enzyme detection: suitable

pH值

<1 (20 °C in H2O)

轉變溫度

flash point 36 °C

密度

0.92 g/cm3 at 20 °C

應用

environmental
food and beverages

儲存溫度

2-8°C

適合性

Enterobacter spp.
Escherichia coli

一般說明

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

應用

Bactident™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

聯結

Replaces: 1.11350.0007

分析報告

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

法律資訊

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

96.8 °F

閃點（°C）

36 °C

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