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表单
solid
质量水平
效能
1000 mg/kg LD50, oral (Rat)
pH值(酸碱度)
6-7 (20 °C, 10 g/L in H2O)
mp
265 °C (decomposition)
溶解性
10 g/L
堆积密度
430 kg/m3
应用
diagnostic assay manufacturing
hematology
histology
储存温度
2-30°C
InChI
1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
InChI key
KSCQDDRPFHTIRL-UHFFFAOYSA-N
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一般描述
Auramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining. Pretreatment of the specimens with Sputofluol™ dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol™ also has a disinfectant effect, with the result that any microorganisms that are present are killed off. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.
应用
for microscopy
分析说明
Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (buffer pH 6.0)431 - 433 nmSpec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; buffer pH 6.0; calc. on dried substance)1100 - 1300TLC-Testpasses testLoss on drying (110 °C)≤ 5 %Suitability for microscopypasses test
法律信息
SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany
警示用语:
Warning
危险分类
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
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