L040050

Cap A

configured for (ÄKTA^® and OligoPilot^®)

• UNSPSC代码: 12352200
• NACRES: NA.21

属性

• 类型: for DNA synthesis
• 质量水平: 200
• 产品线: Proligo Reagents
• 表单: liquid
• 储存条件: dry at room temperature
• 杂质: ≤100 ppm water content (Karl Fischer)
• 颜色: clear
• 适用性: conforms to structure (GC, Formulation)
• 相容性: configured for (ÄKTA^® and OligoPilot^®)
• 储存温度: room temp

说明

应用

Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

制备说明

乙腈/N-甲基咪唑·8/2 (v/v)

其他说明

Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.

法律信息

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全信息

• 象形图: GHS02,GHS06,GHS08,GHS05
• 警示用语：: Danger
• 危险声明: H225,H302 + H332,H311,H314,H361d
• 预防措施声明: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危险分类: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
• 储存分类代码: 3 - Flammable liquids
• WGK: WGK 2
• 闪点(°F): 35.6 °F
• 闪点(°C): 2 °C