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Merck

L3022

Sigma-Aldrich

LB 肉汤(Lennox)

Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

别名:

Luria Bertani肉汤, 伦诺克斯肉汤

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About This Item

UNSPSC代码:
41106200
NACRES:
NA.85

等级

Molecular Biology

质量水平

无菌性

non-sterile

表单

powder

组成

NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

技术

microbiological culture: suitable

pH值(酸碱度)

6.8-7.2(2% solution)

应用

food and beverages
microbiology

储存温度

room temp

适用性

nonselective for Escherichia coli
nonselective for coliforms

一般描述

Lennox LB是一个被高度引用的用于 大肠杆菌培养的微生物生长培养基。这种富含营养的微生物肉汤含有肽、氨基酸和低盐配方的碳水化合物。

应用

Luria肉汤被用于大肠杆菌(Escherichia coli)的维持和繁殖。 大肠杆菌在Luria肉汤中生长得更快,因为胰蛋白胨和酵母为其提供了必需的生长因子,从而无需大肠杆菌自身合成。由于含有NaCl成分,Luria肉汤还含有转运和渗透平衡所必需的电解质。
在三鞭毛虫细胞提取物的分级研究中,它被用于梨形四膜虫胞级分的重悬。
适用于 大肠杆菌 菌株的非选择性培养,用于克隆、生产DNA质粒和生产重组蛋白。 也适用于添加适当抗生素时的选择性培养,包括需要低盐条件的抗生素,如吉欧霉素®

特点和优势

Lennox LB粉末可提供:
  • 便捷式小包装,无需称重步骤
  • 使用大包装可轻松放大规模
  • 一种预算较低的液体替代品
  • 标准配方

制备说明

1.将20 g悬浮于1L蒸馏水中。
2.121 °C下高压灭菌15分钟。
制备LB肉汤:添加1 g葡萄糖,然后按照上述说明操作。
制备Enquist &Sternberg培养基:高压蒸汽灭菌后,以无菌操作加入10 mL无菌1M硫酸镁。

法律信息

Zeocin is a registered trademark of Cayla Sarl

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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J Benavente et al.
International journal of biological macromolecules, 103, 758-763 (2017-05-27)
The preparation of silver nanoparticles (AgNPs) and their incorporation into the structure of a regenerated cellulose membrane by dip coating is presented. Morphological characterization of the AgNPs (average diameter of 20±2nm) was carried out by SEM/TEM, while elastic, electrical and
Francisco Dávila et al.
The Journal of experimental biology, 221(Pt 6) (2018-02-16)
Bacteria can damage sperm and thus reduce the reproductive success of both males and females; selection should therefore favour the evolution of antimicrobial protection. Eusocial hymenopterans might be particularly affected by such bacterial infections because of their mating ecology. In
Margot Doberva et al.
Frontiers in microbiology, 8, 1152-1152 (2017-07-12)
Quorum sensing (QS) is a density-dependent mechanism allowing bacteria to synchronize their physiological activities, mediated by a wide range of signaling molecules including
Bor Kavčič et al.
Nature communications, 11(1), 4013-4013 (2020-08-13)
Antibiotics that interfere with translation, when combined, interact in diverse and difficult-to-predict ways. Here, we explain these interactions by "translation bottlenecks": points in the translation cycle where antibiotics block ribosomal progression. To elucidate the underlying mechanisms of drug interactions between
I M Ogunade et al.
Journal of dairy science, 100(3), 1780-1794 (2017-01-04)
Inhibiting the growth of Escherichia coli O157:H7 (EC) in feeds may prevent the transmission or cycling of the pathogen on farms. The first objective of this study was to examine if addition of propionic acid or microbial inoculants would inhibit

实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

General protocols for growth of competent cells in microbial medium.

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