I5131
IgG from sheep serum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Sinónimos:
Sheep IgG
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About This Item
Productos recomendados
origen biológico
sheep
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
grado
reagent grade
Ensayo
≥95% (SDS-PAGE)
Formulario
essentially salt-free, lyophilized powder
isotipo
IgG
solubilidad
saline: 1 mg/mL at -20 °C (Repeated freezing and thawing is not recommended.)
temp. de almacenamiento
2-8°C
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Descripción general
IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds.
Aplicación
Purified IgG may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, immunoprecipitation and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid-phase immunoadsorbents.
Acciones bioquímicas o fisiológicas
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
IgG mediates transplacental transport and participates in the secondary immune response. IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be linked to allergy. It has the longest serum half life.
Nota de análisis
Protein concentration should be determined after reconstitution by absorbance at 280 nm (E1%/280 = 14).
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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