The molecular weight of this product is not determined on a lot to lot basis. According to published literature, the molecular weight of Immunoglobulin G (IgG) is approximately 150 - 160 KDa. Historical data available for this product indicates that in SDS-PAGE electrophoresis this material will have multiple bands ranging from 155 kDa - 220 kDa.
I4506
IgG from human serum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Sinónimos:
IgG humana
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About This Item
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conjugado
unconjugated
Nivel de calidad
grado
reagent grade
Ensayo
≥95% (SDS-PAGE)
Formulario
essentially salt-free, lyophilized powder
composición
Protein, ≥90%
técnicas
ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable
temp. de almacenamiento
2-8°C
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Categorías relacionadas
Descripción general
La IgG humana purificada se aísla de suero combinado humano sano por fraccionamiento y cromatografía de intercambio iónico. El producto se suministra como un polvo liofilizado esencialmente exento de sal (menos del 1 % de sodio).
Aplicación
La IgG del suero humano se utilizó como patrón a una concentración de 1274 mg/ml en ELISA para determinar las respuestas de anticuerpos a Porphyromonas gingivalis y a Prevotella intermedia.[1] Se utilizó para el inmunomarcaje de las células Vero, BHK y PtK2 a una concentración de 0,2 mg/ml.[2] La IgG del suero humano se utilizó como patrón en la SDS-PAGE de anticuerpos contra el LPS de Shigella.[3]
Acciones bioquímicas o fisiológicas
El subtipo de anticuerpo IgG es el más abundante de las inmunoglobulinas séricas del sistema inmunitario. Las IgG son secretadas por las células B y se encuentran en la sangre y los líquidos extracelulares; proporcionan protección frente a infecciones causadas por bacterias, hongos y virus. Las IgG maternas son transferidas al feto a través de la placenta, que es vital para la defensa inmunitaria del neonato frente a las infecciones.[4]
Cláusula de descargo de responsabilidad
Salvo que se especifique lo contrario en nuestro catálogo o en otra documentación de la empresa que acompañe al producto, nuestros productos están indicados sólo para investigación y no deben utilizarse para ningún otro propósito, como, entre otros, usos comerciales no autorizados, diagnóstico in vitro, usos terapéuticos ex vivo o in vivo o cualquier tipo de consumo o aplicación en humanos o animales.
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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What is the expected MW of the product?
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Necesito saber la secuencia de la proteína o el número para buscarla en uniprot
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The protein sequence is not determined for this product. Human IgG has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. These are present in serum at approximately 66%, 23%, 7% and 4%, respectively.
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What is the isoelectric point of this
1 answer-
This product is not tested on a lot-to-lot basis to determine the isoelectric point. However, historical data indicates an isoelectric point range of 6.1 - 8.5.
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Is product I4506, because it is derived from human serum, considered to potential biohazard?
1 answer-
As indicated in the corresponding Data Sheet at the link below: "BIOHAZARD: Handle as if capable of transmitting infectious agents". The source material is tested and found negative for antibody to HIV, antibody to HCV, and for HbsAG.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdfHelpful?
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What is the significance of absorbance at 280 nm (E280 1% = 14)?
1 answer-
The absorbance at 280 nm (E280 1% = 14) indicates the percent extinction coefficient (ϵ1%).
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What is the recommended storage method for item I4506, IgG from human serum, after reconstitution?
1 answer-
After reconstitution, keep aliquots at 2-8°C for short-term use (up to 4 weeks) and store the remaining aliquots at -20°C.
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what is the expected molecular weight under reducing conditions
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I4506, Human IgG, is a tetrameric protein consisting of 2 light chains and 2 heavy. When run on a reducing gel, The heavy chain will be detectable at ~50kDa, and the light chain at ~25kda.
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How can I use Product I4506, IgG from human serum, to block Fc receptors on the cell surface for flow cytometry analysis of cells?
1 answer-
Human cells to be used for FACS staining may be Fc-blocked by treatment with 1 μg of human IgG per 100,000 cells, for 15 minutes at room temperature. It is not necessary to wash to remove excess IgG before proceeding with staining.
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How should I store my solution of Product I4506, IgG from human serum?
1 answer-
Solutions can be stored at -20 °C for at least 1 year.
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Is Product I4506, IgG from human serum, tested for endotoxin?
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No. Product No. I4506 is not tested for endotoxin levels.
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