Saltar al contenido
Merck
Todas las fotos(1)

Documentos

C7594

Sigma-Aldrich

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

Sinónimos:

blocking buffer

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

EC Number:
232-555-1
UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

form

powder blend

technique(s)

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

storage temp.

room temp

Categorías relacionadas

General description

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Application

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

Preparation Note

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

related product

Referencia del producto
Descripción
Precios

P7059

Disolución salina tamponada con fosfato, 10× PBS for Western blots and IP

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

H7140

Hybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

M5755

MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers

H7033

PerfectHyb™ Plus Hybridization Buffer, for Northern and Southern blotting, solution

D9905

Denhardt′s Solution, for Northern and Southern blotting, lyophilized powder

D2532

Denhardt′s Solution 50x, for Northern and Southern blotting, solution

P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

85639

SSC Buffer, for Northern and Southern blotting, powder blend

93017

SSC Buffer 20x, for Northern and Southern blotting, solution

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)
Serapion Pyrpassopoulos et al.
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Elizabeth R Sunderhaus et al.
Frontiers in neuroscience, 13, 1207-1207 (2019-11-30)
Mutations in patatin-like phospholipase domain-containing protein 6 (PNPLA6) have been linked with a number of inherited diseases with clinical symptoms that include spastic paraplegia, ataxia, and chorioretinal dystrophy. PNPLA6 is an evolutionary conserved protein whose ortholog in Drosophila is Swiss-Cheese
Ver todos los artículos relacionados

Protocolos

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico