Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

M5755

Sigma-Aldrich

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Sinónimos:

MESA Buffer

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

grado

for molecular biology

Nivel de calidad

200

Línea del producto

BioReagent

Formulario

powder

técnicas

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubilidad

H2O: soluble, faintly yellow

actividad extraña

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

temp. de almacenamiento

room temp

Categorías relacionadas

Descripción general

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Aplicación

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Componentes

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)

Reconstitución

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Producto relacionado

Referencia del producto
Descripción
Precios

D9156

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

D0428

1 kb DNA Ladder, for DNA electrophoresis

H7033

PerfectHyb™ Plus Hybridization Buffer, for Northern and Southern blotting, solution

T9285

Tampón Tris-EDTA, 100X concentrate for Northern and Southern blotting

S6639

SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

B6185

Bionic™ Buffer, 10X Concentrate, for Northern and Southern blotting, solution

GERPN3005

ECL™ Direct Nucleic Acid, Cytiva RPN3005, pack of 1 ea

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000383887
0000257463
0000344083
0000257463
0000344083

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Los clientes también vieron

Slide 1 of 3

1 of 3

MOPS sodium salt anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD018

MOPS sodium salt

MOPS, Free Acid, Molecular Biology Grade CAS 1132-61-2 is a zwitterionic buffer useful in the pH range of 6.5-7.9. Has a pKa of 7.20 at 25°C.

Millipore

475922

MOPS, Free Acid, Molecular Biology Grade

MOPS anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD003

MOPS

Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 7-7 (1989)
Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
Xueyu Xiang et al.
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Electrophoresis in agarose and acrylamide gels.
R C Ogden et al.
Methods in enzymology, 152, 61-87 (1987-01-01)
Ver todos los artículos relacionados

Protocolos

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico