Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

NB20

Sigma-Aldrich

Anti-AnkyrinG Mouse mAb (463)

liquid, ≥95% (SDS-PAGE), clone 463

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

463, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, mouse, human, canine

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ANK3(288)

General description

Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of AnkyrinG.
Recognizes the ~190 kDa ankyrinG protein in brain extracts. Reacts with all spliced forms of ankyrinG.
  • Antibody Target Gene Symbol: ANK3
  • Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
  • Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
  • Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057)
  • Mu Entrez ID: 11735
  • Rat Entrez ID: 361833

    • Immunogen

    Human
    a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG

    Application


    Frozen Sections (1-2 g/ml)
    Immunoblotting (2-5 g/ml)
    Immunofluorescence (1-2 g/ml)

    Packaging

    Please refer to vial label for lot-specific concentration.

    Warning

    Toxicity: Standard Handling (A)

    Physical form

    In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

    Analysis Note

    Positive Control
    Mouse or rat brain extract or unmyelinated axons

    Other Notes

    AnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
    Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement.
    Kunimoto, M., et al. 1995. FEBS Lett.357, 217.
    Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.
    Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.
    Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.
    Otto, E., et al. 1991. J. Cell Biol.114, 241.
    Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    ¿No encuentra el producto adecuado?  

    Pruebe nuestro Herramienta de selección de productos.

    Storage Class

    10 - Combustible liquids

    wgk_germany

    nwg

    flash_point_f

    Not applicable

    flash_point_c

    Not applicable

    Certificados de análisis (COA)

    Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

    ¿Ya tiene este producto?

    Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

    Visite la Librería de documentos

    Dhaval Nanavati et al.
    Journal of neurochemistry, 119(3), 617-629 (2011-08-16)
    Bipolar disorder is a devastating illness that is marked by recurrent episodes of mania and depression. There is growing evidence that the disease is correlated with disruptions in synaptic plasticity cascades involved in cognition and mood regulation. Alleviating the symptoms
    Hongyan Zhu et al.
    Scientific reports, 6, 38108-38108 (2016-12-03)
    Voltage-gated sodium channels (VGSCs) play a vital role in controlling neuronal excitability. Nav1.6 is the most abundantly expressed VGSCs subtype in the adult central nervous system and has been found to contribute to facilitate the hyperexcitability of neurons after electrical
    Ruani N Fernando et al.
    Nature communications, 7, 12186-12186 (2016-07-21)
    Fast nerve conduction relies on successive myelin segments that electrically isolate axons. Segment geometry-diameter and length-is critical for the optimization of nerve conduction and the molecular mechanisms allowing this optimized geometry are partially known. We show here that peripheral myelin
    Wing Yan Yu et al.
    Stem cells and development, 24(5), 624-639 (2014-10-18)
    A growing body of evidence has revealed that stem-like cells in the posterior limbus of the eye between the corneal endothelium (CE) and trabecular meshwork (TM) may be able to rejuvenate these tissues in disease. However, these cells have not

    Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

    Póngase en contacto con el Servicio técnico