Accéder au contenu
Merck
Toutes les photos(4)

Key Documents

View All Documentation

U4002

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type IX, powder, 50,000-100,000 units/g solid

Synonyme(s) :

Jack bean urease, Urea amidohydrolase

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(4)

About This Item

Numéro CAS:
9002-13-5
Numéro de classification (Commission des enzymes):
3.5.1.5 (BRENDA, IUBMB)
Numéro CE :
232-656-0
Numéro MDL:
MFCD00070858
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54

Type

Type IX

Niveau de qualité

200

Forme

powder

Activité spécifique

50,000-100,000 units/g solid

Poids mol.

~544620 Da

Température de stockage

2-8°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.

Application

Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis.

Actions biochimiques/physiologiques

In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline.
Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.

Définition de l'unité

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Isothermal titration calorimetry to characterize enzymatic reactions
Methods in Enzymology, 567, 215-236 (2016)
Armored Urease: Enzyme-Bioconjugated Poly(acrylamide) Hydrogel as a Storage and Sensing Platform
Kunduru KR, et al.
Test, 590, 143-167 (2017)
An investigation of the use of urease-antibody conjugates in enzyme immunoassays
Chandler HM, et al.
Journal of Immunological Methods, 53(2), 187-194 (1982)
Xavier Arqué et al.
ACS nano, 16(5), 7547-7558 (2022-04-30)
The increasing resistance of bacteria to existing antibiotics constitutes a major public health threat globally. Most current antibiotic treatments are hindered by poor delivery to the infection site, leading to undesired off-target effects and drug resistance development and spread. Here
G A Rosenthal et al.
Plant physiology, 76(2), 541-544 (1984-10-01)
l-Canavanine, the guanidinooxy structural analog of l-arginine, is an important nonprotein amino acid of many leguminous plants with nitrogen storage a major proported role. l-[Guanidinooxy-(14)C]canavanine, [(14)C] urea, and [(15)N]urea were injected separately into the fleshy, green cotyledons of 9-day old
Afficher tous les articles scientifiques apparentés

Articles

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique