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Merck
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Key Documents

U1500

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type III, powder, 15,000-50,000 units/g solid

Synonyme(s) :

Jack bean urease, Urea amidohydrolase

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54

Source biologique

Canavalia ensiformis

Niveau de qualité

Type

Type III

Forme

powder

Activité spécifique

15,000-50,000 units/g solid

Poids mol.

~544620 Da

Application(s)

diagnostic assay manufacturing

Température de stockage

2-8°C

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Description générale

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.

Actions biochimiques/physiologiques

Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.

Définition de l'unité

1 U setzt 1.0 μmol NH3 aus Harnstoff pro Minute bei pH 7.0 und 25 °C frei. Es ist äquivalent zu 1.0 I.U. oder 0.054 Sumner Einheiten (1.0 mg Ammoniak Stickstoff in 5 Minuten bei pH 7.0 und 20 °C)

Pictogrammes

Health hazardExclamation mark

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1


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Fernanda Stanisçuaski et al.
Journal of insect physiology, 55(3), 255-263 (2009-01-06)
Ureases are metalloenzymes that are widespread among plants, fungi and bacteria. Urease isoforms (jack bean urease-JBU and canatoxin) from Canavalia ensiformis seeds are toxic to insects and fungi, suggesting a role in plant defense. The entomotoxic effect is due to
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Journal of microbiology and biotechnology, 31(12), 1684-1691 (2021-12-29)
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